Zhang Xiaoping, Shu Miaoan, Wang Yibing, Fu Luoqin, Li Weifen, Deng Bin, Liang Quan, Shen Wenying
College of Animal Science, Zhejiang University, Hangzhou, 310058, People's Republic of China.
World J Microbiol Biotechnol. 2014 Sep;30(9):2523-31. doi: 10.1007/s11274-014-1677-1. Epub 2014 May 31.
To investigate the effects of photosynthetic bacteria as additives on water quality, microbial community structure and diversity, a photosynthetic purple non-sulfur bacteria, Rhodopseudamonas palustris, was isolated and used to remove nitrogen in the aquaculture water. The results of water quality showed that the levels of ammonia nitrogen, nitrite nitrogen, total inorganic nitrogen and total nitrogen in the treatment group were significantly lower (p < 0.05) than the nitrogen levels of the controls in an extended range. A 454-pyrosequencing analysis revealed that at the level of phylum, Proteobacteria and Firmicutes were dominant in the control group respectively, compared to the dominance of the phyla Proteobacteria, Bacteroidetes and Actinobacteria in the treatment group. The relative abundance of phyla Bacteroidetes and Actinobacteria in treatment witnessed an increase than that in the control. The results also indicated that the treatment group enjoyed a higher microbial diversity than that of the control group. Based on the oxygen requirement and metabolism, the authors observed that the water supplementation with photosynthetic bacteria could significantly decrease (p < 0.05) the number of nitrite reducer and anaerobic bacteria. Therefore, the results suggested that adding photosynthetic bacteria to water improves the water quality as it changes the microbial community structure.
为研究光合细菌作为添加剂对水质、微生物群落结构和多样性的影响，分离出一株光合紫色非硫细菌沼泽红假单胞菌，并将其用于去除水产养殖水中的氮。水质结果表明，在较长时间范围内，处理组中的氨氮、亚硝酸盐氮、总无机氮和总氮水平均显著低于对照组（p < 0.05）。454焦磷酸测序分析显示，在门水平上，对照组中变形菌门和厚壁菌门分别占主导地位，而处理组中则是变形菌门、拟杆菌门和放线菌门占主导。处理组中拟杆菌门和放线菌门的相对丰度比对照组有所增加。结果还表明，处理组的微生物多样性高于对照组。基于需氧量和代谢情况，作者观察到添加光合细菌的水体中亚硝酸盐还原菌和厌氧菌数量显著减少（p < 0.05）。因此，结果表明向水中添加光合细菌可改善水质，因为它改变了微生物群落结构。
