Arockiaraj Jesu, Gnanam Annie J, Palanisamy Rajesh, Bhatt Prasanth, Kumaresan Venkatesh, Chaurasia Mukesh Kumar, Pasupuleti Mukesh, Ramaswamy Harikrishnan, Arasu Abirami, Sathyamoorthi Akila
Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur 603 203, Chennai, Tamil Nadu, India.
Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A4800, Austin, TX 78712, USA.
Gene. 2014 Aug 10;546(2):437-42. doi: 10.1016/j.gene.2014.05.063. Epub 2014 May 28.
Glutathione S-transferases play an important role in cellular detoxification and may have evolved to protect cells against reactive oxygen metabolites. In this study, we report the molecular characterization of glutathione s-transferase-theta (GST-θ) from freshwater prawn Macrobrachium rosenbergii. A full length cDNA of GSTT (1417 base pairs) was isolated and characterized bioinformatically. Exposure to virus (white spot syndrome baculovirus or M. rosenbergii nodovirus), bacteria (Aeromonas hydrophila or Vibrio harveyi) or heavy metals (cadmium or lead) significantly increased the expression of GSTT (P<0.05) in hepatopancreas. Recombinant GST-θ with monochlorobimane substrate had an optimum activity at pH7.5 and 35 °C. Furthermore recombinant GST-θ activity was abolished by the denaturants triton X-100, Gua-HCl, Gua-thiocyanate, SDS and urea in a dose-dependent manner. Overall, the results suggest a potential role for M. rosenbergii GST-θ in detoxification and possibly conferring immune protection.
谷胱甘肽S-转移酶在细胞解毒过程中发挥着重要作用，可能是为保护细胞免受活性氧代谢产物的侵害而进化产生的。在本研究中，我们报道了罗氏沼虾谷胱甘肽S-转移酶-θ（GST-θ）的分子特征。分离得到了GSTT的全长cDNA（1417个碱基对），并进行了生物信息学表征。暴露于病毒（白斑综合征杆状病毒或罗氏沼虾诺达病毒）、细菌（嗜水气单胞菌或哈维氏弧菌）或重金属（镉或铅）后，肝胰腺中GSTT的表达显著增加（P<0.05）。含单氯联苯胺底物的重组GST-θ在pH7.5和35°C时具有最佳活性。此外，变性剂曲拉通X-100、盐酸胍、硫氰酸胍、十二烷基硫酸钠和尿素以剂量依赖性方式使重组GST-θ的活性丧失。总体而言，结果表明罗氏沼虾GST-θ在解毒过程中可能发挥作用，并可能提供免疫保护。
