Australian Institute for Bioengineering and Nanotechnology (AIBN), Corner College and Cooper Roads (Bldg 75), The University of Queensland, Brisbane, QLD 4072, Australia.
Australian Institute for Bioengineering and Nanotechnology (AIBN), Corner College and Cooper Roads (Bldg 75), The University of Queensland, Brisbane, QLD 4072, Australia.
Biosens Bioelectron. 2014 Nov 15;61:184-91. doi: 10.1016/j.bios.2014.03.061. Epub 2014 May 14.
We report a simple method to remove nonspecifically adsorbed species from sensor surface and also improve the detection sensitivity of the sensor using tuneable alternating current (ac) electrohydrodynamics (ac-EHD) forces. These forces generated within few nanometers of an electrode surface (i.e., double layer) engender fluid flow within a serpentine channel containing a long array of the asymmetric electrode pairs, and can easily be tuned externally by changing the frequency and amplitude of the ac-EHD field. Under the optimized experimental conditions, we achieved a 3.5-fold reduction in nonspecific adsorption of non-target proteins with a 1000-fold enhancement in detection sensitivity of the device for the analysis of human epidermal growth factor receptor 2 (HER2) protein spiked in serum. This approach can be applicable in diverse fields including biosensors, cellular and molecular separation systems and biomedical applications to remove/reduce nonspecific adsorption of molecular and cellular species.
我们报告了一种简单的方法，可利用可调谐交流（ac）电动力学（ac-EHD）力从传感器表面去除非特异性吸附的物质，并提高传感器的检测灵敏度。这些力在电极表面（即双电层）几纳米范围内产生，在包含长阵列不对称电极对的蛇形通道内引起流体流动，并且可以通过改变 ac-EHD 场的频率和幅度轻松地进行外部调节。在优化的实验条件下，我们实现了非目标蛋白质非特异性吸附的 3.5 倍降低，并且该设备对人表皮生长因子受体 2（HER2）蛋白在血清中的分析的检测灵敏度提高了 1000 倍。这种方法可应用于包括生物传感器、细胞和分子分离系统以及生物医学应用在内的多个领域，以去除/减少分子和细胞物质的非特异性吸附。
