Sahiner Fatih, Gümral Ramazan, Yildizoğlu Üzeyir, Babayiğit Mustafa Alparslan, Durmaz Abdullah, Yiğit Nuri, Saraçli Mehmet Ali, Kubar Ayhan
Division of Medical Virology, Department of Medical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey.
Department of Medical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey.
Int J Pediatr Otorhinolaryngol. 2014 Aug;78(8):1288-93. doi: 10.1016/j.ijporl.2014.05.012. Epub 2014 May 21.
In this study, we aimed to investigate the presence and copy number of six different viruses in tonsillar tissue samples removed surgically because of chronic recurrent tonsillitis or chronic obstructive tonsillar hypertrophy.
In total, 56 tissue samples (tonsillar core) collected from 44 children and 12 adults were included in this study. The presence of viruses was investigated using a new TaqMan-based quantitative real-time PCR assay.
Of the 56 tissue samples, 67.9% (38/56) were positive for at least one of the six viruses. Epstein-Barr virus was the most frequently detected virus, being found in 53.6% (30/56), followed by human Parvovirus B19 21.4% (12/56), human adenovirus 12.5% (7/56), human Cytomegalovirus 5.4% (3/56), BK polyomavirus 1.8% (1/56), and Herpes simplex virus 1.8% (1/56). Precancerous or cancerous changes were not detected in the tonsillar tissue samples by pathologic examination, whereas lymphoid hyperplasia was observed in 24 patients. In contrast to other viruses, B19 virus was present in high copy number in tonsillar tissues. The rates of EBV and B19 virus with high copy number (>500.000 copies/ml) were higher in children than in adults, and a positive relationship was also found between the presence of EBV and the presence of B19 virus with high copy number (P=0.037).
It is previously reported that some viral agents are associated with different chronic tonsillar pathologies. In the present study, the presence of B19 virus in tonsillar core samples was investigated quantitatively for the first time, and our data suggests that EBV infections could be associated with B19 virus infections or could facilitate B19 virus replication. However, further detailed studies are needed to clarify this observation.
在本研究中，我们旨在调查因慢性复发性扁桃体炎或慢性阻塞性扁桃体肥大而手术切除的扁桃体组织样本中六种不同病毒的存在情况及拷贝数。
本研究共纳入了从44名儿童和12名成人收集的56份组织样本（扁桃体核心）。使用基于TaqMan的新型定量实时PCR检测法来调查病毒的存在情况。
在56份组织样本中，67.9%（38/56）至少对六种病毒中的一种呈阳性。爱泼斯坦-巴尔病毒是最常检测到的病毒，占53.6%（30/56），其次是人类细小病毒B19，占21.4%（12/56），人类腺病毒占12.5%（7/56），人类巨细胞病毒占5.4%（3/56），BK多瘤病毒占1.8%（1/56），单纯疱疹病毒占1.8%（1/56）。病理检查未在扁桃体组织样本中检测到癌前或癌变变化，而在24名患者中观察到淋巴组织增生。与其他病毒不同，B19病毒在扁桃体组织中的拷贝数很高。儿童中EBV和高拷贝数（>500,000拷贝/毫升）的B19病毒的发生率高于成人，并且还发现EBV的存在与高拷贝数的B19病毒的存在之间存在正相关关系（P=0.037）。
先前有报道称某些病毒因子与不同的慢性扁桃体病变有关。在本研究中，首次对扁桃体核心样本中B19病毒的存在情况进行了定量研究，我们的数据表明EBV感染可能与B19病毒感染有关或可能促进B19病毒复制。然而，需要进一步详细研究来阐明这一观察结果。
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