Kalantari Shirin, Zadheidar Sevrin, Heydarifard Zahra, Nejati Ahmad, Sadeghi Kaveh, Shatizadeh Malekshahi Somayeh, Ghavami Nastaran, Mokhtari-Azad Talat, Shafiei-Jandaghi Nazanin-Zahra
Department of Virology, School of Public Health Tehran University of Medical Sciences Tehran Iran.
Department of Virology, Faculty of Medical Sciences Tarbiat Modares University Tehran Iran.
World J Otorhinolaryngol Head Neck Surg. 2024 Jan 3;10(4):270-274. doi: 10.1002/wjo2.156. eCollection 2024 Dec.
Epstein-Barr virus (EBV) infection is ubiquitous all around the world. Tonsils seem to be candidate replication sites for EBV, and these tissues can be infected acutely or chronically. Some studies reported an association between EBV infection and tonsillar hypertrophy. In this study, we aimed to evaluate the presence and copy number of the EBV genome in tonsil tissue specimens of patients with tonsillar hypertrophy.
A cross-sectional study was performed on 50 fresh tonsil tissue samples from children, who underwent tonsillectomy because of tonsillar hypertrophy. Patients' tonsil tissues were evaluated using real-time polymerase chain reaction for EBV genome and viral load. Finally, the results were analyzed using SPSS software.
EBV genome was detected in 58% (29/50) of tonsillar tissues. The relationship between EBV genome detection rate and age groups was in the statistical significance range ( = 0.051). Among 29 positive cases, the average EBV viral load was (3.1 × 10) copy/g ± (0.5 × 10) copy/g. No significant difference was observed among different sex and age groups for EBV viral load.
Herein, EBV genome detection could support the colonization of EBV in the tonsils, which may have a direct or indirect association with the pathogenesis of tonsillar hypertrophy.
爱泼斯坦-巴尔病毒（EBV）感染在全球普遍存在。扁桃体似乎是EBV的候选复制位点，这些组织可被急性或慢性感染。一些研究报道了EBV感染与扁桃体肥大之间的关联。在本研究中，我们旨在评估扁桃体肥大患者扁桃体组织标本中EBV基因组的存在情况及拷贝数。
对50例因扁桃体肥大接受扁桃体切除术的儿童新鲜扁桃体组织样本进行了一项横断面研究。使用实时聚合酶链反应评估患者的扁桃体组织中的EBV基因组和病毒载量。最后，使用SPSS软件分析结果。
在58%（29/50）的扁桃体组织中检测到EBV基因组。EBV基因组检测率与年龄组之间的关系在统计学显著范围内（P = 0.051）。在29例阳性病例中，EBV平均病毒载量为（3.1×10）拷贝/克±（0.5×10）拷贝/克。不同性别和年龄组的EBV病毒载量未观察到显著差异。
在此，EBV基因组检测可支持EBV在扁桃体中的定植，这可能与扁桃体肥大的发病机制有直接或间接关联。
