α-降钙素基因相关肽（α-CGRP）介导3T3-L1脂肪细胞中的脂质动员。
α-Calcitonin gene related peptide (α-CGRP) mediated lipid mobilization in 3T3-L1 adipocytes.
作者信息
Walker Christopher S, Hay Debbie L, Fitzpatrick Sandra M, Cooper Garth J S, Loomes Kerry M
机构信息
School of Biological Sciences, University of Auckland, Auckland, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland, New Zealand.
School of Biological Sciences, University of Auckland, Auckland, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland, New Zealand.
出版信息
Peptides. 2014 Aug;58:14-9. doi: 10.1016/j.peptides.2014.05.011. Epub 2014 Jun 2.
The neuropeptide, α-calcitonin gene-related peptide (α-CGRP), is expressed from sensory nerves that innervate fat. However, how α-CGRP may act in adipose tissue is unclear. Using 3T3-L1 adipocytes we observed that rat α-CGRP (rα-CGRP) evoked either a biphasic or monophasic reduction in intracellular free fatty acid (FFA) content. cAMP production was always monophasic and occurred when FFA responses were absent. Taken together with the observed potencies, these findings suggest that adipose tissue is a physiological target for α-CGRP. However, uncoupling of the FFA and CGRP-signaling responses with increasing passage number limits 3T3-L1 adipocytes as a suitable cellular model.
神经肽α-降钙素基因相关肽（α-CGRP）由支配脂肪的感觉神经表达。然而，α-CGRP在脂肪组织中如何发挥作用尚不清楚。利用3T3-L1脂肪细胞，我们观察到大鼠α-CGRP（rα-CGRP）引起细胞内游离脂肪酸（FFA）含量呈双相或单相降低。环磷酸腺苷（cAMP）的产生总是单相的，且在没有FFA反应时发生。结合观察到的效力，这些发现表明脂肪组织是α-CGRP的生理靶点。然而，随着传代次数增加，FFA和CGRP信号反应的解偶联限制了3T3-L1脂肪细胞作为合适的细胞模型。
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