Kikuchi S, Aosaki T, Bito K, Naito S, Katayama Y
Analytical Science Research Laboratories, Kao Corporation, Tochigi, Japan.
Skin Res Technol. 2015 Feb;21(1):76-83. doi: 10.1111/srt.12159. Epub 2014 Jun 3.
BACKGROUND/AIMS: The matrix of intercellular lipids (ICL) of stratum corneum (SC) plays an important role in the barrier function of SC. It is important to understand the structure of the ICL matrix for dermatology and cosmetic science. Several methods exist for the analysis of the structure; however, it is difficult to conduct these analyses noninvasively.
We have developed a method for the analysis of the lateral packing of ICL using Raman spectroscopy. As a proof-of-principle experiment, we prepared a human SC sheet sample and analyzed its structure by the proposed method and by a conventional method involving X-ray diffraction. We compared the results of both methods. In addition, we applied the proposed method to living human skin, and we analyzed the lateral packing of ICL of SC taken from three separate body sites.
The results of our method corresponded to those of the conventional method. We detected regional differences of ICL lateral packing using our method in vivo. The results indicated that the packing of ICL in SC taken from the forearm and upper arm are more ordered than that taken from the cheek.
The results verify that our developed method allows the evaluation of the lateral packing of ICL inside the SC layer of the skin in vivo. Using this method, we can detect regional differences of SC samples taken from various body sites.
背景/目的：角质层（SC）的细胞间脂质（ICL）基质在SC的屏障功能中起重要作用。了解ICL基质的结构对于皮肤病学和化妆品科学至关重要。存在几种分析该结构的方法；然而，难以进行非侵入性分析。
我们开发了一种使用拉曼光谱分析ICL侧向堆积的方法。作为原理验证实验，我们制备了人SC薄片样品，并通过所提出的方法和涉及X射线衍射的传统方法分析其结构。我们比较了两种方法的结果。此外，我们将所提出的方法应用于活体人类皮肤，并分析了取自三个不同身体部位的SC的ICL侧向堆积。
我们方法的结果与传统方法的结果一致。我们使用我们的方法在体内检测到ICL侧向堆积的区域差异。结果表明，取自前臂和上臂的SC中ICL的堆积比取自脸颊的更有序。
结果证实我们开发的方法能够在体内评估皮肤SC层内ICL的侧向堆积。使用这种方法，我们可以检测取自不同身体部位的SC样品的区域差异。
