Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Charitéplatz 1, 10117 Berlin, Germany.
Kim Il Sung University, Ryongnam-Dong, Taesong District, Pyongyang, DPR Korea.
J Biophotonics. 2018 Jun;11(6):e201700355. doi: 10.1002/jbio.201700355. Epub 2018 Apr 10.
Porcine skin is widely used as a human skin model in dermatology. For both, porcine stratum corneum (SC) ex vivo and human SC in vivo, the hydrogen bonding states of water, the secondary and tertiary structures of keratin, the natural moisturizing factor (NMF) concentrations and the intercellular lipids' (ICL) lateral organization are investigated depth-dependently using confocal Raman microscopy. The SC depth profiles show that porcine SC ex vivo is characterized by lower hydrogen bonding states of water (10%-30% SC depth), lower NMF concentration in the whole SC, more β-sheet form of keratin (10%-90% SC depth), more folded tertiary keratin structures (30%-70% SC depth) and higher hexagonal lateral packing order of ICL (10%-50% SC depth) compared to human SC in vivo. The results clearly show a higher value of skin barrier function of human SC in vivo than of porcine SC ex vivo. Thus, the human SC in vivo is less permeable for lipophilic and hydrophilic substances than porcine SC ex vivo. Considering the porcine SC as an ex vivo model of human SC in vivo, these findings should be taken into consideration.
猪皮在皮肤科中被广泛用作人体皮肤模型。对于猪的表皮角质层（SC）离体和人体 SC 活体，我们使用共聚焦拉曼显微镜深度依赖地研究了水的氢键状态、角蛋白的二级和三级结构、天然保湿因子（NMF）浓度和细胞间脂质（ICL）的侧向组织。SC 深度分布显示，猪的 SC 离体的特点是水的氢键状态较低（10%-30%SC 深度）、整个 SC 中的 NMF 浓度较低、角蛋白的 β-折叠形式较多（10%-90%SC 深度）、三级角蛋白结构折叠较多（30%-70%SC 深度）以及 ICL 的六方侧向堆积有序度较高（10%-50%SC 深度），与人体 SC 活体相比。这些结果清楚地表明，人体 SC 活体的皮肤屏障功能高于猪的 SC 离体。因此，与猪的 SC 离体相比，人体 SC 活体对亲脂性和亲水性物质的渗透性较低。考虑到猪的 SC 作为人体 SC 活体的离体模型，应该考虑到这些发现。
