Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou, 510640 People's Republic of China ; Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, No. 2 Nengyuan Road, Tianhe District, Guangzhou, 510640 People's Republic of China ; University of Chinese Academy of Sciences, Beijing, 100049 People's Republic of China.
Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou, 510640 People's Republic of China ; Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, No. 2 Nengyuan Road, Tianhe District, Guangzhou, 510640 People's Republic of China.
Indian J Microbiol. 2014 Sep;54(3):268-73. doi: 10.1007/s12088-014-0450-3. Epub 2014 Feb 7.
In this work, acetic acid was found as one promising substrate to improve xylose utilization by Gluconacetobacter xylinus CH001. Also, with the help of adding acetic acid into medium, the bacterial cellulose (BC) production by G. xylinus was increased significantly. In the medium containing 3 g l(-1) acetic acid, the optimal xylose concentration for BC production was 20 g l(-1). In the medium containing 20 g l(-1) xylose, the xylose utilization and BC production by G. xylinus were stimulated by acetic acid within certain concentration. The highest BC yield (1.35 ± 0.06 g l(-1)) was obtained in the medium containing 20 g l(-1) xylose and 3 g l(-1) acetic acid after 14 days. This value was 6.17-fold higher than the yield (0.21 ± 0.01 g l(-1)) in the medium only containing 20 g l(-1) xylose. The results analyzed by FE-SEM, FTIR, and XRD showed that acetic acid affected little on the microscopic morphology and physicochemical characteristics of BC. Base on the phenomenon observed, lignocellulosic acid hydrolysates (xylose and acetic acid are main carbon sources present in it) could be considered as one potential substrate for BC production.
在这项工作中，发现乙酸是一种有前途的底物，可以提高木醋杆菌 CH001 对木糖的利用。此外，通过在培养基中添加乙酸，可以显著提高木醋杆菌的细菌纤维素（BC）产量。在含有 3 g/L 乙酸的培养基中，BC 生产的最佳木糖浓度为 20 g/L。在含有 20 g/L 木糖的培养基中，木糖利用率和 BC 生产受到一定浓度乙酸的刺激。在含有 20 g/L 木糖和 3 g/L 乙酸的培养基中，经过 14 天，木醋杆菌获得了最高的 BC 产率（1.35±0.06 g/L）。这一数值是仅含有 20 g/L 木糖的培养基中（0.21±0.01 g/L）产率的 6.17 倍。FE-SEM、FTIR 和 XRD 的分析结果表明，乙酸对 BC 的微观形貌和物理化学特性影响不大。基于观察到的现象，木质纤维素酸水解物（木糖和乙酸是其中主要的碳源）可以被视为 BC 生产的一种潜在底物。
