小麦根际相关不动杆菌菌株 HIRFA32 产铁载体的特性及其抑菌活性研究。
Characterization and Fungal Inhibition Activity of Siderophore from Wheat Rhizosphere Associated Acinetobacter calcoaceticus Strain HIRFA32.
机构信息
Institute of Bioinformatics and Biotechnology, University of Pune, Pune, 411007 India.
Department of Chemistry, University of Pune, Pune, 411007 India.
出版信息
Indian J Microbiol. 2014 Sep;54(3):315-22. doi: 10.1007/s12088-014-0446-z. Epub 2014 Jan 29.
Abstract
Acinetobacter calcoaceticus HIRFA32 from wheat rhizosphere produced catecholate type of siderophore with optimum siderophore (ca. 92 % siderophore units) in succinic acid medium without FeSO4 at 28 °C and 24 h of incubation. HPLC purified siderophore appeared as pale yellow crystals with molecular weight [M(+1)] m/z 347.18 estimated by LCMS. The structure elucidated by (1)H NMR, (13)C NMR, HMQC, HMBC, NOESY and decoupling studies, revealed that siderophore composed of 2,3-dihydroxybenzoic acid with hydroxyhistamine and threonine as amino acid subunits. In vitro study demonstrated siderophore mediated mycelium growth inhibition (ca. 46.87 ± 0.5 %) of Fusarium oxysporum. This study accounts to first report on biosynthesis of acinetobactin-like siderophore by the rhizospheric strain of A. calcoaceticus and its significance in inhibition of F. oxysporum.
摘要
小麦根际产碱菌 HIRFA32 产生邻苯二酚型铁载体，在不含 FeSO4 的琥珀酸培养基中于 28°C 下孵育 24 小时，最佳产铁载体（约 92%铁载体单位）。HPLC 纯化的铁载体呈淡黄色晶体，通过 LCMS 估计分子量 [M(+1)] m/z 为 347.18。通过 (1)H NMR、(13)C NMR、HMQC、HMBC、NOESY 和去耦研究阐明了铁载体的结构，由 2,3-二羟基苯甲酸与羟组氨酸和苏氨酸组成氨基酸亚基。体外研究表明铁载体介导的丝核菌菌丝生长抑制（约 46.87±0.5%）。本研究首次报道了根际产碱菌合成类似阿丁菌素的铁载体及其在抑制尖孢镰刀菌中的意义。
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