林木相关细菌产生的针对多种植物病原真菌的可扩散性和挥发性有机化合物

Forest Tree Associated Bacterial Diffusible and Volatile Organic Compounds against Various Phytopathogenic Fungi.

作者信息

Kong Wei-Liang, Li Pu-Sheng, Wu Xiao-Qin, Wu Tian-Yu, Sun Xiao-Rui

机构信息

Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China.

Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing 210037, China.

出版信息

Microorganisms. 2020 Apr 18;8(4):590. doi: 10.3390/microorganisms8040590.

Plant growth-promoting rhizobacteria (PGPR) can potentially be used as an alternative strategy to control plant diseases. In this study, strain ST-TJ4 isolated from the rhizosphere soil of a healthy poplar was found to have a strong antifungal activity against 11 phytopathogenic fungi in agriculture and forestry. Strain ST-TJ4 was identified as sp. based on 16S rRNA-encoding gene sequences. The bacterium can produce siderophores, cellulase, and protease, and has genes involved in the synthesis of phenazine, 1-phenazinecarboxylic acid, pyrrolnitrin, and hydrogen cyanide. Additionally, the volatile compounds released by strain ST-TJ4 can inhibit the mycelial growth of plant pathogenic fungi more than diffusible substances can. Based on volatile compound profiles of strain ST-TJ4 obtained from headspace collection and GC-MS/MS analysis, 1-undecene was identified. In summary, the results suggested that sp. ST-TJ4 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi.

植物促生根际细菌（PGPR）有潜力作为控制植物病害的一种替代策略。在本研究中，从健康杨树根际土壤中分离出的菌株ST-TJ4被发现对11种农林植物病原真菌具有很强的抗真菌活性。基于16S rRNA编码基因序列，菌株ST-TJ4被鉴定为属。该细菌能产生铁载体、纤维素酶和蛋白酶，并具有参与吩嗪、1-吩嗪羧酸、硝吡咯菌素和氰化氢合成的基因。此外，菌株ST-TJ4释放的挥发性化合物比可扩散物质更能抑制植物病原真菌的菌丝生长。基于从顶空收集和GC-MS/MS分析获得的菌株ST-TJ4的挥发性化合物谱，鉴定出1-十一碳烯。总之，结果表明属的ST-TJ4菌株可作为由植物病原真菌引起的各种植物病害的生物防治剂。