Role of lipid peroxidation products, plasma total antioxidant status, and Cu-, Zn-superoxide dismutase activity as biomarkers of oxidative stress in elderly prediabetics.

UNLABELLED

The relationship between hyperglycemia and oxidative stress in diabetes is well known, but the influence of metabolic disturbances recognized as prediabetes, in elderly patients especially, awaits for an explanation.

METHODS

52 elderly persons (65 years old and older) with no acute or severe chronic disorders were assessed: waist circumference (WC), body mass index (BMI), percentage of body fat (FAT), and arterial blood pressure. During an oral glucose tolerance test (OGTT) fasting (0') and 120-minute (120') glycemia and insulinemia were determined, and type 2 diabetics (n = 6) were excluded. Subjects were tested for glycated hemoglobin HbA1c, plasma lipids, total antioxidant status (TAS), thiobarbituric acid-reacting substances (TBARS), and activity of erythrocyte superoxide dismutase (SOD-1). According to OGTT results, patients were classified as normoglycemics, (NGT, n = 18) and prediabetics, (PRE, n = 28).

RESULTS

Both groups did not differ with their lipids, FAT, and TBARS. PRE group had higher WC (P < 0.002) and BMI (P < 0.002). Lower SOD-1 activity (P < 0.04) and TAS status (P < 0.04) were found in PRE versus NGT group.

SIGNIFICANCE

In elderly prediabetics, SOD-1 and TAS seem to reflect the first symptoms of oxidative stress, while TBARS are later biomarkers of oxidative stress.