Consiglio Nazionale delle Ricerche (CNR), Istituto di Scienze dell'Alimentazione, via Roma 64, I-83100 Avellino, Italy.
J Agric Food Chem. 2014 Jun 25;62(25):5853-8. doi: 10.1021/jf5025533. Epub 2014 Jun 17.
Donkey milk has been recently revalued intensely due to its nutritional properties. Moreover, donkey milk has been proposed as an effective alternative food for some infants with cow milk allergy. Two fast analytical methods were proposed to detect the fraudulent practice of blending cow milk to donkey milk. Detection of cow αs1-casein bands along the profiles of experimental donkey-cow milk mixtures analyzed by isoelectric focusing was adequate to estimate cow milk used as adulterant of donkey milk starting from 5% (v/v). An ELISA-based method using the antipeptide antibodies raised against the 1-28 sequence stretch of cow β-casein was also developed for an accurate definition of composition of donkey-cow milk mixtures. The presence of cow milk at levels as low as 0.5% (v/v) was detected in donkey-cow milk mixtures prepared at laboratory scale and assayed by ELISA.
驴奶的营养价值最近受到了高度重视。此外，驴奶已被提议作为一些对牛奶过敏的婴儿的有效替代食品。本文提出了两种快速分析方法来检测将牛奶混入驴奶的欺诈行为。通过等电聚焦分析实验性驴-牛混合乳的图谱，可以检测到牛αs1-酪蛋白带，足以估计从 5%（v/v）开始用作驴奶掺杂物的牛奶。还开发了一种基于 ELISA 的方法，使用针对牛β-酪蛋白 1-28 序列片段的抗肽抗体，用于准确定义驴-牛混合乳的组成。通过 ELISA 检测，在实验室规模制备的驴-牛混合乳中，即使牛奶含量低至 0.5%（v/v）也能检测到。
