Department of Health Science, Magna Græcia University, Viale Europa, loc. Germaneto, 88100, Catanzaro, Italy.
Department of Health Science, Magna Græcia University, Viale Europa, loc. Germaneto, 88100, Catanzaro, Italy.
J Dairy Sci. 2019 Jul;102(7):5962-5970. doi: 10.3168/jds.2018-16194. Epub 2019 May 2.
The addition of cow milk during the production of buffalo mozzarella is a common fraud in dairy industries because of the lower price and greater availability of cow milk throughout the year. The aim of this study was to develop a new, rapid, and robust capillary electrophoresis method for detecting and quantifying cow milk in buffalo milk by exploiting cow α-lactalbumin as a marker of adulteration. In particular, a linear calibration curve was generated, using a training set of calibrators consisting of 7 series of 17 buffalo/bovine whey mixtures, obtained after casein precipitation, with increasing percentages of cow whey. The capillary electrophoresis method showed high linearity (R = 0.968), repeatability [relative standard deviation (RSD) = 2.11, 3.02, 4.38, and 1.18%, respectively for 5, 10, 20, and 50% of buffalo/bovine whey mixtures], and intermediate precision (RSD = 2.18, 2.49, 5.09, and 3.19%, respectively, for 5, 10, 20, and 50% buffalo/bovine whey mixtures). Moreover, the minimum amount of detectable fraudulent cow milk was 1%, and the limit of quantification was 3.1%.
在水牛乳马苏里拉奶酪的生产过程中添加牛奶是乳制品行业常见的欺诈行为，因为牛奶的价格更低，而且全年供应更充足。本研究旨在开发一种新的、快速且稳健的毛细管电泳方法，通过利用牛奶α-乳白蛋白作为掺假标记物来检测和定量水牛乳中的牛奶。具体来说，使用由 7 个系列组成的校准品集（每个系列由 17 个水牛/牛乳清混合物组成），通过酪蛋白沉淀后获得，其中包含不同百分比的牛乳清，生成线性校准曲线。毛细管电泳法表现出高线性度（R = 0.968）、重复性[相对标准偏差（RSD）分别为 2.11%、3.02%、4.38%和 1.18%，用于 5%、10%、20%和 50%的水牛/牛乳清混合物]和中间精密度（RSD 分别为 2.18%、2.49%、5.09%和 3.19%，用于 5%、10%、20%和 50%的水牛/牛乳清混合物）。此外，可检测到的欺诈性牛奶的最小量为 1%，定量限为 3.1%。
