Gidoh M, Tsutsumi S
Nihon Rai Gakkai Zasshi. 1989 Oct-Dec;58(4):241-9. doi: 10.5025/hansen1977.58.241.
The inhibitory effects by the combined doses of bacteriostatic DDS and several immunostimulants on the growth of leprosy bacilli (LB) inoculated into the footpads of nude mice were examined, using a strain of hybrid nude mice named Jcl:AF-nu, which is resistible to infections more than strain BALB/c-nu. The results found were: 1. LB could proliferate up to 10(9) level in the footpads of this strain of hybrid nude mice. 2. The combined dose of muramyl dipeptide (MDP) or a water-soluble lipoidal amine, CP-46665 and DDS mixed with chow in the content ratio of 0.005% completely inhibited the growth of LB. Whereas, the growth inhibition by DDS alone was only partial. 3. A derivative of MDP, muroctasin could partially inhibit the growth of LB without combined dose of DDS. 4. The combined dose of an antitumor beta-1, 3-glucan named ATSO could not enhance the partial growth inhibition due to the dose of DDS through 0.005%-DDS chow. Based on these results, the possibility of Jcl:AF-nu mice as a favorable animal model for examining the synergic inhibitory effect on the growth of LB due to the combined dose of an antileprous chemotherapeutic and an immunostimulant was discussed.
使用一种名为Jcl:AF-nu的杂交裸鼠品系，研究了抑菌性二氨基二苯砜（DDS）与几种免疫刺激剂联合使用对接种于裸鼠脚垫的麻风杆菌（LB）生长的抑制作用，该品系杂交裸鼠比BALB/c-nu品系更能抵抗感染。研究结果如下：1. 在该品系杂交裸鼠的脚垫中，LB可增殖至10⁹水平。2. 胞壁酰二肽（MDP）或水溶性类脂胺CP-46665与DDS按0.005%的含量比例与饲料混合的联合剂量可完全抑制LB的生长。而单独使用DDS时，生长抑制只是部分性的。3. MDP的衍生物muroctasin在不与DDS联合使用时可部分抑制LB的生长。4. 一种名为ATSO的抗肿瘤β-1,3-葡聚糖的联合剂量不能增强通过含0.005% DDS的饲料中DDS剂量所产生的部分生长抑制作用。基于这些结果，讨论了Jcl:AF-nu小鼠作为一种合适的动物模型用于研究抗麻风化疗药物与免疫刺激剂联合剂量对LB生长的协同抑制作用的可能性。
