Verderio Paolo, Bottelli Stefano, Ciniselli Chiara Maura, Pierotti Marco Alessandro, Gariboldi Manuela, Pizzamiglio Sara
Unit of Medical Statistics, Biometry, and Bioinformatics, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy.
Unit of Medical Statistics, Biometry, and Bioinformatics, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy.
Anal Biochem. 2014 Sep 15;461:7-9. doi: 10.1016/j.ab.2014.05.020. Epub 2014 Jun 2.
In this note, we propose an R function named NqA (Normalization qPCR Array, where qPCR is quantitative real-time polymerase chain reaction) suitable for the identification of a set of microRNAs (miRNAs) to be used for data normalization in view of subsequent validation studies with qPCR data. NqA is available through the website of the Fondazione IRCCS Istituto Nazionale dei Tumori of Milan (http://www.istitutotumori.mi.it/modules.php?name=Content&pa=showpage&pid=812) with a dedicated user's guide. We applied our function on a qPCR dataset downloaded from the Gene Expression Omnibus (GEO) database. Results show that NqA provides a functional subset of reference miRNAs and a set of promising significantly modulated miRNAs for subsequent validation studies.
在本笔记中，我们提出了一个名为NqA（标准化qPCR阵列，其中qPCR是定量实时聚合酶链反应）的R函数，鉴于后续对qPCR数据的验证研究，该函数适用于鉴定一组用于数据标准化的微小RNA（miRNA）。可通过米兰Fondazione IRCCS Istituto Nazionale dei Tumori的网站（http://www.istitutotumori.mi.it/modules.php?name=Content&pa=showpage&pid=812）获取NqA，并配有专门的用户指南。我们将我们的函数应用于从基因表达综合数据库（GEO）下载的一个qPCR数据集。结果表明，NqA为后续验证研究提供了一组参考miRNA的功能子集以及一组有前景的显著调控的miRNA。
