Esteve-Turrillas Francesc A, Mercader Josep V, Agulló Consuelo, Marzo Javier, Abad-Somovilla Antonio, Abad-Fuentes Antonio
Institute of Agrochemistry and Food Technology, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Agustí Escardino 7, 46980 Paterna, València, Spain.
Analyst. 2014 Jul 21;139(14):3636-44. doi: 10.1039/c3an01104f.
Boscalid is a modern agrochemical belonging to the so-called chemical class of succinate dehydrogenase inhibitor fungicides. With the aim of developing rapid analytical screening methods for this relevant compound, we herein report the synthesis of new boscalid mimics and the study of their suitability for the production of polyclonal antibodies. Aliphatic spacer arms equivalent in length and composition were tethered at two different aromatic rings of the target molecular structure. These haptens, besides being used for immunization, were employed in the development of heterologous competitive enzyme-linked immunosorbent assays (cELISAs) in order to improve assay detectability. Direct and indirect immunoassays were tailored and applied to the determination of samples with incurred boscalid residues. The assays were characterized in terms of sensitivity, specificity, trueness, and precision. Limit of quantification was established at 5 μg kg(-1), coefficients of variation were lower than 20%, and recoveries from spiked samples ranged from 90 to 137%. Finally, ELISA performance was evaluated by Deming regression analysis with tomato and cucumber samples, selecting ultra-performance liquid chromatography-mass spectrometry as the reference method. The results showed that the proposed cELISAs are useful for the routine determination of boscalid fungicides in foods with high-sample throughput and affordable cost.
啶酰菌胺是一种现代农用化学品，属于琥珀酸脱氢酶抑制剂类杀菌剂这一所谓的化学类别。为了开发针对这种相关化合物的快速分析筛选方法，我们在此报告新型啶酰菌胺模拟物的合成及其用于生产多克隆抗体的适用性研究。在目标分子结构的两个不同芳环上连接了长度和组成相当的脂肪族间隔臂。这些半抗原除了用于免疫外，还用于开发异源竞争性酶联免疫吸附测定法（cELISA），以提高测定的可检测性。定制了直接和间接免疫测定法，并将其应用于含有啶酰菌胺残留的样品的测定。对这些测定法的灵敏度、特异性、准确性和精密度进行了表征。定量限设定为5 μg kg⁻¹，变异系数低于20%，加标样品的回收率在90%至137%之间。最后，通过Deming回归分析，以番茄和黄瓜样品评估ELISA性能，并选择超高效液相色谱 - 质谱法作为参考方法。结果表明，所提出的cELISA对于食品中啶酰菌胺类杀菌剂的常规测定很有用，具有高样品通量和可承受的成本。
