Department of Biotechnology, Institute of Agrochemistry and Food Technology, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Agustí Escardino 7, 46980 Paterna, València, Spain.
Department of Organic Chemistry, Universitat de València, Doctor Moliner 50, 46100 Burjassot, València, Spain.
Food Chem. 2018 Nov 30;267:2-9. doi: 10.1016/j.foodchem.2017.06.013. Epub 2017 Jun 3.
Boscalid is an agrochemical recently developed for crop protection and the most significant member of the succinate dehydrogenase inhibitor group of fungicides. In this study, a collection of high-affinity monoclonal antibodies was generated to boscalid. By using a series of haptens with a linker at alternative tethering sites of the boscalid framework, specific antibodies were isolated as well as antibodies that also recognized the main boscalid metabolite. Two immunoassays were developed using different ELISA formats. Optimized assays displayed very high sensitivities (limits of detection were near 0.01µg/L). Trueness and precision for the determination of the target analyte in strawberry samples was evaluated. Moreover, immunoassay performance was validated with a reference chromatographic method using QuEChERS extracts of fruits from fungicide-treated crops. A monitoring study with strawberry samples from local markets was carried out by immunoassay, showing an occurrence of boscalid of 15% with a maximum residue concentration of 43µg/kg.
波尔多液是一种最近开发的用于作物保护的农用化学品，是琥珀酸脱氢酶抑制剂类杀菌剂中最重要的成员。本研究针对波尔多液生成了一组高亲和力的单克隆抗体。通过使用一系列带有连接子的半抗原，连接在波尔多液结构的不同连接点上，分离出了特异性抗体，以及能够识别主要波尔多液代谢物的抗体。使用两种不同的 ELISA 格式开发了两种免疫测定法。优化后的测定法具有非常高的灵敏度（检测限接近 0.01µg/L）。对草莓样品中目标分析物的测定进行了真实性和精密度评估。此外，还使用经 QuEChERS 提取的来自经杀菌剂处理的作物的水果的参考色谱方法验证了免疫测定法的性能。通过免疫测定法对当地市场的草莓样品进行了监测研究，结果显示波尔多液的检出率为 15%，最大残留浓度为 43µg/kg。
