Pandey Ramesh Prasad, Gurung Rit Bahadur, Parajuli Prakash, Koirala Niranjan, Tuoi Le Thi, Sohng Jae Kyung
Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, 100 Kalsan-ri, Tangjeonmyun, Asan-si, Chungnam 336-708, Republic of Korea.
Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, 100 Kalsan-ri, Tangjeonmyun, Asan-si, Chungnam 336-708, Republic of Korea.
Carbohydr Res. 2014 Jul 1;393:26-31. doi: 10.1016/j.carres.2014.03.011. Epub 2014 May 9.
The acceptor substrate promiscuity of YjiC, a UDP-glycosyltransferase from Bacillus licheniformis, was explored with seven different classes (flavonols, flavanols, flavones, flavanones, chalcone, stilbene, and isoflavonoids) of 23 flavonoid acceptors. For most of the polyphenols used in the reactions, the enzymatic bioconversion was significantly higher with the production of multiple glucosylated derivatives. This study highlights the highly flexible non-regiospecific glycosylation ability of YjiC toward polyphenolic compounds. The catalytic potential of YjiC could be useful to generate a library of natural product glucosides.
利用来自地衣芽孢杆菌的UDP-糖基转移酶YjiC，对23种黄酮类受体的7种不同类别（黄酮醇、黄烷醇、黄酮、黄烷酮、查耳酮、芪和异黄酮）进行了受体底物混杂性研究。对于反应中使用的大多数多酚，酶促生物转化显著更高，产生了多种糖基化衍生物。这项研究突出了YjiC对多酚类化合物具有高度灵活的非区域特异性糖基化能力。YjiC的催化潜力可能有助于生成天然产物糖苷文库。
