Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University, Tangjeong-myeon, Asan-si, Chungnam, Republic of Korea.
Appl Environ Microbiol. 2013 Nov;79(21):6833-8. doi: 10.1128/AEM.02057-13. Epub 2013 Aug 23.
The glycosylation of five different flavonols, fisetin, quercetin, myricetin, kaempferol, and 3-hydroxyflavone, was achieved by applying YjiC. 3-Hydroxyflavone was selected as a probe for in vitro glycorandomization of all flavonols using diverse nucleotide diphosphate-d/l-sugars. This study unlocked the possibilities of the glycodiversification of flavonols and the generation of novel compounds as future therapeutics.
五种不同的黄酮醇，即非瑟酮、槲皮素、杨梅素、山奈酚和 3-羟基黄酮，通过应用 YjiC 进行了糖基化。选择 3-羟基黄酮作为探针，使用不同的核苷酸二磷酸-d/l-糖，对所有黄酮醇进行体外糖基随机化。这项研究为黄酮醇的糖基多样化以及生成新化合物作为未来治疗方法提供了可能。
