Kiraz Hasan A, Erbas Mesut, Kucuk Adem, Topaloglu Naci, Erdem Havva, Sahin Hasan, Toman Huseyin, Kiraz Aslı
Department of Anesthesiology and Reanimation, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey.
Department of Pediatric Surgery, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey.
Adv Clin Exp Med. 2015 Jul-Aug;24(4):637-41. doi: 10.17219/acem/22364.
Corrosive esophagitis injuries are a serious clinical problem with many agents used for diagnosis and treatment. Experimental esophagus burn models use a method described mainly in studies by Gehanno and Guedon, and modified by Liu and Richardson.
The aim of this study was to describe a new esophagitis model that prevents tracheal aspiration.
In this study we used 16 Wistar albino rats weighing between 220-240 g. The experimental animals were randomly divided into two groups: the model group (group M, n: 8) and control group (group C, n: 8). The necessary anesthesia was administered. Passing through a median laparotomy incision, the abdomen was entered and in group M and C the esophagus was freed and held by a suture at the gastroesophageal junction. After this procedure, about 1 cm proximal to the gastroesophageal junction, the esophagus was suspended by a suture. The esophagus segment between the two sutures was exposed to 0.1 mL 10% NaOH in group M and 0.1 mL saline in group C for 20 s. Ten days later all experimental animals were sacrificed and their esophagus removed. After dying with hematoxylin and eosine trichrome, the histopathological evaluation results for the rats in all groups were investigated with a light microscope.
Histopathological examination indicated submucosal collagen increase, damage to muscularis mucosa and tunica muscularis and collagen deposition. In the model group, the rats had high neutrophils and tissue damage accompanied by necrosis. In the control group, the rats had minimal or no tissue damage and fibrosis was not observed.
Our procedure is relatively less invasive and easy to apply with corrosive esophagitis only in the required region, and at the same time treatment medications can be easily administered.
腐蚀性食管炎损伤是一个严重的临床问题，有多种用于诊断和治疗的药物。实验性食管烧伤模型采用的方法主要是Gehanno和Guedon研究中所描述的，并经Liu和Richardson修改。
本研究的目的是描述一种可防止气管误吸的新型食管炎模型。
本研究使用了16只体重在220 - 240克之间的Wistar白化大鼠。将实验动物随机分为两组：模型组（M组，n = 8）和对照组（C组，n = 8）。给予必要的麻醉。通过正中剖腹切口进入腹腔，在M组和C组中游离食管，并在胃食管交界处用缝线固定。此操作后，在胃食管交界处近端约1厘米处，用缝线悬吊食管。M组在两根缝线之间的食管段暴露于0.1毫升10%氢氧化钠中20秒，C组暴露于0.1毫升生理盐水中20秒。10天后，处死所有实验动物并取出其食管。用苏木精和伊红三色染色后，用光学显微镜研究所有组大鼠的组织病理学评估结果。
组织病理学检查显示黏膜下胶原增加、黏膜肌层和肌层损伤以及胶原沉积。模型组大鼠中性粒细胞增多且伴有坏死的组织损伤。对照组大鼠组织损伤最小或无损伤，未观察到纤维化。
我们的方法侵入性相对较小，仅在所需区域易于应用于腐蚀性食管炎，同时治疗药物易于给药。
