Davies C S, Suhrbier A S, Winger L A, Sinden R E
Acta Leiden. 1989;58(2):97-113.
The in vitro exoerythrocytic (EE) of Plasmodium berghei was compared in primary rat and mouse hepatocytes and the human hepatoma cell line HepG2. All of the cell-types supported the full maturation of EE stages, but the HepG2 cells were much more susceptible to infection than the primary rodent hepatocytes and were also the most efficient host cells. Following refinement of culture techniques, the development of EE forms which is now observed in HepG2 cells closely reflects that occurring in vivo with respect to the morphology and size of parasites and their rate of maturation. Furthermore, high densities of parasites are reproducibly achieved. A detailed description is presented of exoerythrocytic development in HepG2 cells. The application of these cultures to chemosensitivity studies is discussed and the relative advantages of employing cell lines or primary hepatocytes as host cells in such a system are considered.
在原代大鼠和小鼠肝细胞以及人肝癌细胞系HepG2中对伯氏疟原虫的体外红细胞外期（EE）进行了比较。所有细胞类型均支持EE期的完全成熟，但HepG2细胞比原代啮齿动物肝细胞更容易受到感染，并且也是最有效的宿主细胞。随着培养技术的改进，现在在HepG2细胞中观察到的EE形式的发育在寄生虫的形态和大小及其成熟速率方面与体内发生的情况密切相关。此外，可重复获得高密度的寄生虫。本文详细描述了HepG2细胞中的红细胞外期发育。讨论了这些培养物在化学敏感性研究中的应用，并考虑了在这样的系统中使用细胞系或原代肝细胞作为宿主细胞的相对优势。
