Sinden R E, Suhrbier A, Davies C S, Fleck S L, Hodivala K, Nicholas J C
Department of Pure and Applied Biology, Imperial College, London, England.
Bull World Health Organ. 1990;68 Suppl(Suppl):115-25.
Methods are reviewed for the culture of the exoerythrocytic stages of Plasmodium berghei wherein development reproducibly reflects growth observed in vivo in laboratory rodents. The combination of these methods with the culture of both asexual and sexual blood stages has allowed the completion of the entire vertebrate phase of malaria development in vitro. The development of new methods for high-density exoerythrocytic-stage culture combined with robust statistical analysis of parasite growth by morphological (light microscopy), or DNA probe methods now allows the critical and precise evaluation of chemotherapeutic or immunological treatments. These methods are illustrated by data obtained on pyrimethamine, primaquine and a hydroxynaphthoquinone. Some of the new avenues of research made feasible by the high-density cultures, e.g., direct immunization to produce monoclonal antibodies and biochemical studies are discussed.
本文回顾了伯氏疟原虫红细胞外期培养的方法，该期的发育可重复性地反映了实验室啮齿动物体内观察到的生长情况。这些方法与无性和有性血液期培养相结合，使得疟疾发育的整个脊椎动物阶段能够在体外完成。高密度红细胞外期培养新方法的开发，结合通过形态学（光学显微镜）或DNA探针方法对寄生虫生长进行的稳健统计分析，现在能够对化疗或免疫治疗进行关键而精确的评估。本文通过乙胺嘧啶、伯氨喹和羟基萘醌的数据说明了这些方法。讨论了高密度培养可行的一些新研究途径，例如直接免疫以产生单克隆抗体和生化研究。
