Mohammedi H, Mamouzi S, Allal C, Ghaffor M, Rabhi H, Abbadi M C
Arch Inst Pasteur Alger. 1989;57:151-62.
The Coomassie blue method of proteins quantitation initially reported by Bradford and modified by Macart et al. appears to be simple, fast, sensitive and of low cost. In this study, we report an improved micromethod derived from the technic described by Macart. Applied to the protein quantitation of 100 cerebrospinal fluid (CSF) specimens and 50 serum specimens our method showed a very good correlation with the macromethod of Macart (r = 0.985 for CSF proteins, r = 0.944 for serum proteins) and also with the Lowry technic (r = 0.986 for CSF proteins, r = 0.898 for serum proteins). Our technic may be used with various biological fluids (urines, tears, saliva...) and seems to be of particular interest for low protein level fluids and for little volume samples (it needs only 20 mcl).
最初由布拉德福德报道并经马卡特等人修改的考马斯亮蓝蛋白质定量方法似乎简单、快速、灵敏且成本低。在本研究中，我们报告了一种源自马卡特所描述技术的改进微量方法。应用于100份脑脊液（CSF）标本和50份血清标本的蛋白质定量，我们的方法与马卡特的宏观方法显示出非常好的相关性（CSF蛋白质的r = 0.985，血清蛋白质的r = 0.944），并且与洛瑞技术也有很好的相关性（CSF蛋白质的r = 0.986，血清蛋白质的r = 0.898）。我们的技术可用于各种生物体液（尿液、眼泪、唾液等），对于低蛋白水平的体液和少量样本（仅需20微升）似乎特别有用。
