用考马斯亮蓝G - 250测定琼脂糖结合蛋白。
Determination of Sepharose-bound protein with Coomassie brilliant blue G-250.
作者信息
Asryants R A, Duszenkova I V, Nagradova N K
出版信息
Anal Biochem. 1985 Dec;151(2):571-4. doi: 10.1016/0003-2697(85)90222-2.
PMID:4096388
Abstract
The colorimetric procedure of Bradford (M.M. Bradford, 1976, Anal. Biochem. 72, 248-254) was found to be convenient for determining the content of a protein immobilized on Sepharose. Being simple, sensitive, and rapid, this method appears very useful in studies involving multiple analyses of immobilized protein species present at low concentrations.
摘要
人们发现，Bradford的比色法（M.M. Bradford，1976年，《分析生物化学》第72卷，第248 - 254页）便于测定固定在琼脂糖凝胶上的蛋白质含量。该方法简单、灵敏且快速，在涉及对低浓度存在的固定化蛋白质种类进行多次分析的研究中似乎非常有用。
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