Krzemień D, Klimek M
Katedry Diagnostyki Biochemicznej AM w Krakowie.
Przegl Lek. 1990;47(2):271-4.
Determination of urine total protein concentration employing Macart and Gerbaut method, with rapid assessment of urine protein with Ames strip method was performed and the results were compared. Studied material consisted of a set urines from healthy persons and urines with proteinuria obtained from Outpatient Clinic of Nephrology and Clinic of Metabolic Diseases. In each urine protein was determined using two compared methods. The obtained mean protein concentration in urines with negative deep-stick test was in CBB-SDS method 0.186 +/- 0.112 g/l, whereas for with "trace" protein the CBB-SDS method yields 0.315 +/- 0.159 g/l. For urines with protein concentration assessed as (+), CBB-SDS method yielded 0.558 +/- 0.257 g/l, and for (++), ( ) and ( +) the CBB-SDS method yielded 1.204 +/- 0.454 g/l, 1.647 +/- 0.293 g/l and 2.298 +/- 1.316 g/l respectively. Correlation between protein concentration determined by the CBB-SDS method and deep-stick test Ames method was characterized by r = 0.87 (significant at p less than 0.001). Moreover it was shown correlation between the urine protein concentration determined using both compared methods and number of casts detected in sediment r = 0.33, p less than 0.001 and r = 0.32, p less than 0.001 respectively. On the other hand the Ames Labstix method showed correlation with erythrocytes number, what was not shown using the CBB-SDS method.
采用马卡特和热尔博方法测定尿总蛋白浓度，并通过艾姆斯试纸法快速评估尿蛋白，然后对结果进行比较。研究材料包括一组来自健康人的尿液以及从肾脏病门诊和代谢疾病门诊获取的蛋白尿尿液。对每份尿液使用两种对比方法测定蛋白质。在深试纸条检测为阴性的尿液中，考马斯亮蓝 - 十二烷基硫酸钠（CBB - SDS）法测得的平均蛋白浓度为0.186±0.112 g/l，而对于“微量”蛋白，CBB - SDS法测得的结果为0.315±0.159 g/l。对于蛋白浓度评估为（+）的尿液，CBB - SDS法测得的结果为0.558±0.257 g/l，对于（++）、（+++）和（++++）的尿液，CBB - SDS法测得的结果分别为1.204±0.454 g/l、1.647±0.293 g/l和2.298±1.316 g/l。CBB - SDS法测定的蛋白浓度与艾姆斯深试纸条检测法之间的相关性特征为r = 0.87（p小于0.001时具有显著性）。此外，还显示了使用两种对比方法测定的尿蛋白浓度与尿沉渣中检测到的管型数量之间的相关性，分别为r = 0.33，p小于0.001和r = 0.32，p小于0.001。另一方面，艾姆斯实验室试纸法显示与红细胞数量相关，而CBB - SDS法未显示此相关性。
