Department of Pharmacology, Siddhartha Institute of Pharmacy, Dehradun, India.
Department of Pharmacognosy, College of Dental Medicines and Pharmacy, Buraydah, Saudi Arabia.
J Antibiot (Tokyo). 2014 Jul;67(7):499-504. doi: 10.1038/ja.2014.35. Epub 2014 Jun 4.
It is a well-known fact that to bring a new molecule it may take more than a decade. The existing drugs, which are known for their adverse reaction or toxicity, if utilized and allowed in different formulation, the new effective formulation may be discovered and developed. This may help in reducing various side effects, time and costs. In this study, fungal infection was inoculated superficially over the skin of guinea pigs and treated with the broad-spectrum antimicrobial (gatifloxacin) in combination with non-toxic and effective amount of copper ions. MIC of copper (0.20%) was also determined. Concentration of gatifloxacin (100 μg ml(-1)) with the combination of copper ions (MIC) at which it inhibits the visible growth of fungal strains was also evaluated. Hematological parameters, such as total leukocyte count and differential leukocyte count, were evaluated. The results have shown increase in these parameters after fungal infection, which reaches its normal value after treatment with the combination of gatifloxacin and copper ions. Outcomes of the research concluded that gatifloxacin 100 μg ml(-1) can be used by 0.20% of copper ions to prevent growth of some fungal strains (Candida albicans and Aspergillus niger), which causes skin infections with more potency.
众所周知，将一种新的分子引入临床应用可能需要十多年的时间。现有的药物，如果以不同的配方使用并允许使用，可能会发现并开发出新的有效配方，这些药物已知具有不良反应或毒性。这有助于减少各种副作用、时间和成本。在这项研究中，将真菌感染浅部接种于豚鼠皮肤，并使用广谱抗菌药（加替沙星）联合无毒且有效的铜离子进行治疗。还确定了铜的 MIC（0.20%）。还评估了加替沙星（100μg/ml）浓度与铜离子（MIC）的组合，即抑制真菌菌株可见生长的浓度。评估了血液学参数，如总白细胞计数和白细胞分类计数。结果表明，真菌感染后这些参数增加，用加替沙星和铜离子联合治疗后恢复正常。研究结果表明，加替沙星 100μg/ml 可以与 0.20%的铜离子一起使用，以更有效地预防一些引起皮肤感染的真菌菌株（白色念珠菌和黑曲霉）的生长。
