Hirayama Junichi, Fujihara Mitsuhiro, Akino Mitsuaki, Kojima Shunsuke, Yanagisawa Ryu, Homma Chihiro, Kato Toshiaki, Ikeda Hisami, Azuma Hiroshi, Shimodaira Shigetaka, Takamoto Shigeru
Japanese Red Cross Hokkaido Block Blood Center, Sapporo, Japan.
Transfusion. 2014 Dec;54(12):3173-7. doi: 10.1111/trf.12724. Epub 2014 Jun 4.
Volume-reduced washed platelets (VR-wPLTs), which are prepared by concentrating platelets (PLTs) into a smaller volume of additive solution (AS), may prevent not only circulatory overload, but also adverse reactions caused by plasma components. Although VR-wPLTs may be quickly degraded due to high PLT concentrations, few studies have examined the effects of storage on VR-wPLTs. We examined here the in vitro properties of VR-wPLTs prepared with M-sol AS during their storage for 7 days.
Platelet concentrates (PCs) were divided into two equal aliquots (control group and test group). After the centrifugation of both aliquots and removal of as much supernatant as possible, the pellet of the control group was resuspended in 160 mL of M-sol while that of the test group was resuspended in 80 or 40 mL of M-sol. The wPLTs of both groups were stored in polyolefin bags with agitation at 20 to 24°C for 7 days.
The pH values of both groups were maintained at higher than 7.0 during the 7-day storage. Differences in %disk, CD62P, annexin V, percent hypotonic shock response, and aggregation values between the test group and control group were small for at least 2 days after washing.
The in vitro properties of VR-wPLTs were not markedly degraded for at least 2 days. Therefore, the storage properties of PLTs may be maintained in VR-wPLTs prepared at blood centers until they are administered to patients in hospitals.
体积减少的洗涤血小板（VR-wPLTs）是通过将血小板（PLTs）浓缩到较小体积的添加剂溶液（AS）中制备的，它不仅可以预防循环超负荷，还可以预防血浆成分引起的不良反应。尽管VR-wPLTs可能由于血小板浓度高而迅速降解，但很少有研究考察储存对VR-wPLTs的影响。我们在此研究了用M-sol AS制备的VR-wPLTs在储存7天期间的体外特性。
将血小板浓缩物（PCs）分成两个相等的等分试样（对照组和试验组）。将两个等分试样离心并尽可能去除上清液后，对照组的沉淀重悬于160 mL M-sol中，而试验组的沉淀重悬于80或40 mL M-sol中。两组的洗涤血小板在聚烯烃袋中于20至24°C搅拌储存7天。
在7天的储存期间，两组的pH值均维持在7.0以上。洗涤后至少2天，试验组和对照组之间在盘状率、CD62P、膜联蛋白V、低渗休克反应百分比和聚集值方面的差异很小。
VR-wPLTs的体外特性至少2天内未明显降解。因此，在血液中心制备的VR-wPLTs中血小板的储存特性可能得以维持，直至在医院给患者输注。
