Liu Jinmin, Aye Yimon
University of Oxford, Oxford OX1 3TA, United Kingdom.
ACS Chem Biol. 2025 Mar 21;20(3):539-552. doi: 10.1021/acschembio.4c00835. Epub 2025 Mar 4.
Spurred by the authors' own recent discovery of reactive metabolite-regulated nexuses involving lipid droplets (LDs), this perspective discusses the latest knowledge and multifaceted approaches toward deconstructing the function of these dynamic organelles, LD-associated localized signaling networks, and protein players. Despite accumulating knowledge surrounding protein families and pathways of conserved importance for LD homeostasis surveillance and maintenance across taxa, much remains to be understood at the molecular level. In particular, metabolic stress-triggered contextual changes in LD-proteins' localized functions, crosstalk with other organelles, and feedback signaling loops and how these are specifically rewired in disease states remain to be illuminated with spatiotemporal precision. We hope this perspective promotes an increased interest in these essential organelles and innovations of new tools and strategies to better understand context-specific LD regulation critical for organismal health.
受作者近期发现的涉及脂滴（LDs）的反应性代谢物调节连接的启发，本文探讨了有关解构这些动态细胞器、与LD相关的局部信号网络和蛋白质参与者功能的最新知识和多方面方法。尽管围绕蛋白质家族以及对跨分类群的LD稳态监测和维持具有重要保守意义的途径的知识不断积累，但在分子水平上仍有许多有待了解。特别是，代谢应激引发的LD蛋白局部功能的背景变化、与其他细胞器的串扰以及反馈信号回路，以及这些在疾病状态下如何被特异性重新连接，仍有待以时空精度阐明。我们希望本文能激发人们对这些重要细胞器的更多兴趣，并推动新工具和策略的创新，以更好地理解对机体健康至关重要的特定背景下的LD调节。
