Wang Lu, Xu Fei, Wang Guishuan, Wang Xiaorong, Liang Ajuan, Huang Hefeng, Sun Fei
International Peace Maternity & Child Health Hospital, Shanghai Key laboratory for Reproductive Medicine, School of Medicine, Institute of Embryo-Fetal Original Adult Disease, Shanghai Jiaotong University, Shanghai, 200030, China.
School of Life Sciences, University of Science and Technology of China, Hefei, 230026, China.
Protein Cell. 2016 Oct;7(10):714-721. doi: 10.1007/s13238-016-0308-z. Epub 2016 Sep 8.
Reproduction, fat metabolism, and longevity are intertwined regulatory axes; recent studies in C. elegans have provided evidence that these processes are directly coupled. However, the mechanisms by which they are coupled and the reproductive signals modulating fat metabolism and lifespan are poorly understood. Here, we find that an oogenesis-enriched gene, c30f12.4, is specifically expressed and located in germ cells and early embryos; when the gene is knocked out, oogenesis is disrupted and brood size is decreased. In addition to the reproductive phenotype, we find that the loss of c30f12.4 alters fat metabolism, resulting in decreased fat storage and smaller lipid droplets. Meanwhile, c30f12.4 mutant worms display a shortened lifespan. Our results highlight an important role for c30f12.4 in regulating reproduction, fat homeostasis, and aging in C. elegans, which helps us to better understand the relationship between these processes.
生殖、脂肪代谢和寿命是相互交织的调控轴；最近对秀丽隐杆线虫的研究提供了证据，表明这些过程是直接相关联的。然而，它们相互关联的机制以及调节脂肪代谢和寿命的生殖信号仍知之甚少。在这里，我们发现一个在卵子发生过程中高表达的基因c30f12.4，它在生殖细胞和早期胚胎中特异性表达并定位；当该基因被敲除时，卵子发生受到破坏，产卵量减少。除了生殖表型外，我们还发现c30f12.4的缺失会改变脂肪代谢，导致脂肪储存减少和脂滴变小。同时，c30f12.4突变体线虫的寿命缩短。我们的结果突出了c30f12.4在调控秀丽隐杆线虫的生殖、脂肪稳态和衰老过程中的重要作用，这有助于我们更好地理解这些过程之间的关系。
