Cui-ping Zhang, Shuai Huang, Wen-ting Wei, Shun Ping, Xiao-ge Shen, Ya-jing Li, Fu-liang Hu
College of Animal Sciences, Zhejiang Univ, Nr. 866 Yuhangtang Road, Hangzhou, 310058, China.
J Food Sci. 2014 Jul;79(7):C1315-22. doi: 10.1111/1750-3841.12510. Epub 2014 Jun 3.
A RP-high-performance liquid chromatography (HPLC) method was developed for quality control of Chinese propolis by simultaneous analysis of 12 flavonoids and 8 phenolic acids. The results showed that vanillic acid, rutin, myricetin, and luteolin were not detected in all of the analyzed propolis and poplar tree gum samples. The caffeic acid, ferulic acid and p-coumaric acid were not detected in poplar tree gum but were detected in propolis, which suggest that they are practical indexes of distinguishing propolis from poplar tree gum. The flavonoid profiles of poplar tree gum were found to be similar to those of propolis, which are dominated by pinobanksin, pinocembrin, 3-O-acetylpinobanksin, chrysin, and galangin. Therefore, the proposed method could be applied to exclude poplar tree gum from propolis with cafferic acid, ferulic acid, and p-coumaric acid as qualitative markers, and distinguish poplar source resin from other illegal substances, and evaluate the quality grading of poplar-type propolis with pinobanksin, pinocembrin, 3-O-acetylpinobanksin, chrysin, and galangin as qualitative and quantitative markers.
建立了一种反相高效液相色谱（RP-HPLC）法，通过同时分析12种黄酮类化合物和8种酚酸来对中国蜂胶进行质量控制。结果表明，在所有分析的蜂胶和杨树胶样品中均未检测到香草酸、芦丁、杨梅素和木犀草素。杨树胶中未检测到咖啡酸、阿魏酸和对香豆酸，但在蜂胶中检测到，这表明它们是区分蜂胶和杨树胶的实用指标。发现杨树胶的黄酮类化合物谱与蜂胶相似，主要由白杨素、松属素、3-O-乙酰白杨素、 Chrysin和高良姜素组成。因此，所提出的方法可以以咖啡酸、阿魏酸和对香豆酸作为定性标记物，用于从蜂胶中排除杨树胶，区分杨树源树脂与其他非法物质，并以白杨素、松属素、3-O-乙酰白杨素、 Chrysin和高良姜素作为定性和定量标记物来评估杨树型蜂胶的质量等级。
