Liang Yang, Lin Shi-lei, Wang Cong-wu, Yao Hai-dong, Zhang Zi-wei, Xu Shi-wen
Department of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Biol Trace Elem Res. 2014 Jul;160(1):41-8. doi: 10.1007/s12011-014-0024-6. Epub 2014 Jun 4.
This study describes the effects of selenium (Se) deficiency on the messenger ribonucleic acid (mRNA) expression of 25 selenoproteins (Sels) (including glutathione peroxidases (GPx1-GPx4), thioredoxin reductases (TrxR1-TrxR3), iodothyronine deiodinases (ID1-ID3), selenophosphate synthetase 2 (SPS2), 15-kDa Sel (Sel15), SelH, SelI, SelK, SelM, Sepn1, SelO, Sepx, Selpb, SelS, SelT, SelW, Sepp1, and SelU in the adipose tissues (subcutaneous adipose, visceral adipose, and articular adipose) of chickens. One hundred and fifty 1-day-old chickens were randomly assigned to two groups of 75 each and were fed a low-Se diet (0.032 mg/kg Se) or a control diet (0.282 mg/kg Se). The expression levels of 25 Sel mRNAs were determined on days 35, 45, and 55 from three parts (subcutaneous adipose, visceral adipose, and articular adipose) of the chicken adipose tissues. The results showed that the expression levels of the 25 Sel mRNAs were significantly lower (P < 0.05) in the low-selenium group than in the control group. In addition, the Sel mRNA expression levels in the three adipose tissues were observed to decrease in a time-dependent manner with increasing feeding time.
本研究描述了硒（Se）缺乏对鸡脂肪组织（皮下脂肪、内脏脂肪和关节脂肪）中25种硒蛋白（Sel）（包括谷胱甘肽过氧化物酶（GPx1 - GPx4）、硫氧还蛋白还原酶（TrxR1 - TrxR3）、碘甲状腺原氨酸脱碘酶（ID1 - ID3）、硒磷酸合成酶2（SPS2）、15 kDa硒蛋白（Sel15）、SelH、SelI、SelK、SelM、Sepn1、SelO、Sepx、Selpb、SelS、SelT、SelW、硒蛋白P1（Sepp1）和SelU）信使核糖核酸（mRNA）表达的影响。150只1日龄鸡被随机分为两组，每组75只，分别饲喂低硒日粮（0.032 mg/kg硒）或对照日粮（0.282 mg/kg硒）。在第35、45和55天，测定鸡脂肪组织三个部位（皮下脂肪、内脏脂肪和关节脂肪）中25种Sel mRNA的表达水平。结果表明，低硒组25种Sel mRNA的表达水平显著低于对照组（P < 0.05）。此外，随着饲喂时间的增加，观察到三种脂肪组织中Sel mRNA表达水平呈时间依赖性下降。
