Zhai Chun, Zhang Yu, Yao Nan, Lin Fei, Liu Zhe, Dong Zhongqiu, Wang Ling, Pan Qinghua
National Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China; Guangdong Provincial Key Laboratory for Microbe Signals and Crop Disease Control, South China Agricultural University, Guangzhou, China.
National Key Laboratory of Biocontrol, College of Life Sciences, Sun Yat-sen University, Guangzhou, China.
PLoS One. 2014 Jun 4;9(6):e98067. doi: 10.1371/journal.pone.0098067. eCollection 2014.
Pik-h, an allele of Pik, confers resistance against the rice blast pathogen Magnaporthe oryzae. Its positional cloning has shown that it comprises a pair of NBS-LRR genes, Pikh-1 and Pikh-2. While Pikh-1 appears to be constitutively transcribed, the transcript abundance of Pikh-2 responds to pathogen challenge. The Pikh-1 CC (coiled coil) domain interacts directly with both AvrPik-h and Pikh-2. Transient expression assays demonstrated that Pikh-2 mediates the initiation of the host defence response. Nucleocytoplasmic partitioning of both Pikh-1 and Pikh-2 is required for their functionalities. In a proposed mechanistic model of Pik-h resistance, it is suggested that Pikh-1 acts as an adaptor between AvrPik-h and Pikh-2, while Pikh-2 transduces the signal to trigger Pik-h-specific resistance.
Pik-h是Pik的一个等位基因，赋予对稻瘟病菌Magnaporthe oryzae的抗性。其图位克隆表明它由一对NBS-LRR基因Pikh-1和Pikh-2组成。虽然Pikh-1似乎是组成型转录的，但Pikh-2的转录本丰度对病原体攻击有响应。Pikh-1的CC（卷曲螺旋）结构域直接与AvrPik-h和Pikh-2相互作用。瞬时表达分析表明Pikh-2介导宿主防御反应的启动。Pikh-1和Pikh-2的核质分配是其功能所必需的。在一个提出的Pik-h抗性机制模型中，有人提出Pikh-1作为AvrPik-h和Pikh-2之间的衔接子，而Pikh-2转导信号以触发Pik-h特异性抗性。
