负责Pik-h编码的水稻稻瘟病抗性的耦合基因的功能与相互作用。
Function and interaction of the coupled genes responsible for Pik-h encoded rice blast resistance.
作者信息
Zhai Chun, Zhang Yu, Yao Nan, Lin Fei, Liu Zhe, Dong Zhongqiu, Wang Ling, Pan Qinghua
机构信息
National Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China; Guangdong Provincial Key Laboratory for Microbe Signals and Crop Disease Control, South China Agricultural University, Guangzhou, China.
National Key Laboratory of Biocontrol, College of Life Sciences, Sun Yat-sen University, Guangzhou, China.
出版信息
PLoS One. 2014 Jun 4;9(6):e98067. doi: 10.1371/journal.pone.0098067. eCollection 2014.
Pik-h, an allele of Pik, confers resistance against the rice blast pathogen Magnaporthe oryzae. Its positional cloning has shown that it comprises a pair of NBS-LRR genes, Pikh-1 and Pikh-2. While Pikh-1 appears to be constitutively transcribed, the transcript abundance of Pikh-2 responds to pathogen challenge. The Pikh-1 CC (coiled coil) domain interacts directly with both AvrPik-h and Pikh-2. Transient expression assays demonstrated that Pikh-2 mediates the initiation of the host defence response. Nucleocytoplasmic partitioning of both Pikh-1 and Pikh-2 is required for their functionalities. In a proposed mechanistic model of Pik-h resistance, it is suggested that Pikh-1 acts as an adaptor between AvrPik-h and Pikh-2, while Pikh-2 transduces the signal to trigger Pik-h-specific resistance.
Pik-h是Pik的一个等位基因，赋予对稻瘟病菌Magnaporthe oryzae的抗性。其图位克隆表明它由一对NBS-LRR基因Pikh-1和Pikh-2组成。虽然Pikh-1似乎是组成型转录的，但Pikh-2的转录本丰度对病原体攻击有响应。Pikh-1的CC（卷曲螺旋）结构域直接与AvrPik-h和Pikh-2相互作用。瞬时表达分析表明Pikh-2介导宿主防御反应的启动。Pikh-1和Pikh-2的核质分配是其功能所必需的。在一个提出的Pik-h抗性机制模型中，有人提出Pikh-1作为AvrPik-h和Pikh-2之间的衔接子，而Pikh-2转导信号以触发Pik-h特异性抗性。
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