负责Pik-h编码的水稻稻瘟病抗性的耦合基因的功能与相互作用。

Function and interaction of the coupled genes responsible for Pik-h encoded rice blast resistance.

作者信息

Zhai Chun, Zhang Yu, Yao Nan, Lin Fei, Liu Zhe, Dong Zhongqiu, Wang Ling, Pan Qinghua

机构信息

National Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China; Guangdong Provincial Key Laboratory for Microbe Signals and Crop Disease Control, South China Agricultural University, Guangzhou, China.

National Key Laboratory of Biocontrol, College of Life Sciences, Sun Yat-sen University, Guangzhou, China.

出版信息

PLoS One. 2014 Jun 4;9(6):e98067. doi: 10.1371/journal.pone.0098067. eCollection 2014.

Pik-h, an allele of Pik, confers resistance against the rice blast pathogen Magnaporthe oryzae. Its positional cloning has shown that it comprises a pair of NBS-LRR genes, Pikh-1 and Pikh-2. While Pikh-1 appears to be constitutively transcribed, the transcript abundance of Pikh-2 responds to pathogen challenge. The Pikh-1 CC (coiled coil) domain interacts directly with both AvrPik-h and Pikh-2. Transient expression assays demonstrated that Pikh-2 mediates the initiation of the host defence response. Nucleocytoplasmic partitioning of both Pikh-1 and Pikh-2 is required for their functionalities. In a proposed mechanistic model of Pik-h resistance, it is suggested that Pikh-1 acts as an adaptor between AvrPik-h and Pikh-2, while Pikh-2 transduces the signal to trigger Pik-h-specific resistance.