Iwate Biotechnology Research Center, Kitakami, Iwate, JapanUMR-BGPI, Institut National de la Recherche Agronomique, Montpellier, FranceUMR-BGPI, CIRAD, Montpellier, France.
Plant J. 2012 Dec;72(6):894-907. doi: 10.1111/j.1365-313X.2012.05110.x. Epub 2012 Oct 19.
Attack and counter-attack impose strong reciprocal selection on pathogens and hosts, leading to development of arms race evolutionary dynamics. Here we show that Magnaporthe oryzae avirulence gene AVR-Pik and the cognate rice resistance (R) gene Pik are highly variable, with multiple alleles in which DNA replacements cause amino acid changes. There is tight recognition specificity of the AVR-Pik alleles by the various Pik alleles. We found that AVR-Pik physically binds the N-terminal coiled-coil domain of Pik in a yeast two-hybrid assay as well as in an in planta co-immunoprecipitation assay. This binding specificity correlates with the recognition specificity between AVR and R genes. We propose that AVR-Pik and Pik are locked into arms race co-evolution driven by their direct physical interactions.
攻击和反击对病原体和宿主施加了强烈的相互选择，导致了军备竞赛进化动态的发展。在这里，我们表明稻瘟病菌无毒基因 AVR-Pik 和同源水稻抗性（R）基因 Pik 高度可变，多个等位基因中的 DNA 替换导致氨基酸变化。AVR-Pik 等位基因与各种 Pik 等位基因之间存在紧密的识别特异性。我们发现 AVR-Pik 在酵母双杂交测定以及在植物体内共免疫沉淀测定中物理结合了 Pik 的 N 端卷曲螺旋结构域。这种结合特异性与 AVR 和 R 基因之间的识别特异性相关。我们提出 AVR-Pik 和 Pik 被它们的直接物理相互作用锁定在军备竞赛的共同进化中。
