利用小角散射和标度律探测生物分子的平均局部结构。
Probing the average local structure of biomolecules using small-angle scattering and scaling laws.
机构信息
NIST Center for Neutron Research, National Institute of Standards and Technology, Gaithersburg, Maryland.
出版信息
Biophys J. 2014 Jun 3;106(11):2474-82. doi: 10.1016/j.bpj.2014.03.050.
Abstract
Small-angle neutron and x-ray scattering have become invaluable tools for probing the nanostructure of molecules in solution. It was recently shown that the definite integral of the scattering profile exhibits a scaling (power-law) behavior with respect to molecular mass. We derive the origin of this relationship, and discuss how the integrated scattering profile can be used to identify differing levels of disorder over local ≲30 Å length scales. We apply our analysis to globular and intrinsically disordered proteins.
摘要
小角中子和 X 射线散射已经成为探测溶液中分子纳米结构的非常有价值的工具。最近表明，散射谱的定积分表现出相对于分子质量的标度（幂律）行为。我们推导出这种关系的起源，并讨论如何使用积分散射谱来识别局部 ≲30 Å 长度尺度上不同程度的无序。我们将我们的分析应用于球状和固有无序的蛋白质。
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