Satoh T, Hanazawa S
Meikai Daigaku Shigaku Zasshi. 1989;18(2):187-96.
One monoclonal antibody (BIF6) directed against Bacteroides intermedius Group I was developed by hybridoma technology. In the present study, we used culture medium from BIF6 antibody-producing cells to characterize the antibody. Its reactivity against B. intermedius (Group I, II), B. gingivalis, B. corporis, B. melaninogenicus, B. loescheii, and B. asaccharolyticus was detected by the enzyme-linked immunosorbent assay. BIF6 monoclonal antibody specifically reacted with B. intermedius Group I, but not with B. intermedius Group II. Also, the BIF6 did not react with other black-pigmented Bacteroides. Since BIF6 antibody reacted with some clinical isolates from subgingival plaques of adult periodontal patients, we suggest that this antibody may be useful as a tool in the clinic to identify B. intermedius Group I. Specific antigen of B. intermedius Group I recognized by BIF6 antibody was located on the outer membrane of the organism. And the specific antigen was inactivated by treatment for 20 min at 80 degrees C and at pH2 and 12. Also the antigen was sensitive to trypsin treatment.
通过杂交瘤技术制备了一种针对中间普氏菌I群的单克隆抗体（BIF6）。在本研究中，我们使用产生BIF6抗体的细胞的培养基来鉴定该抗体。通过酶联免疫吸附测定法检测其对中间普氏菌（I群、II群）、牙龈卟啉单胞菌、人体普氏菌、产黑色素普氏菌、洛氏普氏菌和不解糖普氏菌的反应性。BIF6单克隆抗体与中间普氏菌I群特异性反应，但与中间普氏菌II群不反应。此外，BIF6与其他产黑色素普氏菌也不反应。由于BIF6抗体与成年牙周炎患者龈下菌斑中的一些临床分离株发生反应，我们认为该抗体可能作为临床上鉴定中间普氏菌I群的一种工具。BIF6抗体识别的中间普氏菌I群的特异性抗原位于该菌的外膜上。该特异性抗原在80℃、pH2和pH12条件下处理20分钟后失活。该抗原对胰蛋白酶处理也敏感。
