Liang Jinru, He Jiao, Zhu Sha, Zhao Wenna, Zhang Yongmin, Ito Yoichiro, Sun Wenji
Biomedicine Key Laboratory of shaanxi province, Northwest University, Xi'an 710069, China.
Institut Parisien de Chimie Moléculaire, Université Pierre et Marie Curie-Paris 6 (UMR CNRS 7201), 4 place Jussieu, 75005 Paris, France.
J Liq Chromatogr Relat Technol. 2013 Mar 1;36(8):983-999.
Using a two-phase solvent system composed of dichloromethane-methanol-n-butanol-water-acetic acid (5:5:3:4:0.1, ), high-speed countercurrent chromatography was successfully performed for isolation and purification of three iridoid glycosides from Fructus Corni for the first time. From 100 mg of a crude extract of Fructus Corni 7.9 mg of sweroside, 13.1 mg of morroniside, and 10.2 mg of loganin were obtained in less than 3 h with purities of 92.3, 96.3 and 94.2%, respectively. These target compounds were identified by ESI-MS, H NMR and C NMR.
采用由二氯甲烷 - 甲醇 - 正丁醇 - 水 - 乙酸（5:5:3:4:0.1）组成的两相溶剂系统，首次成功地运用高速逆流色谱法从山茱萸中分离纯化出三种环烯醚萜苷。在不到3小时的时间内，从100毫克山茱萸粗提取物中分别获得了7.9毫克獐牙菜苷、13.1毫克莫诺苷和10.2毫克马钱子苷，纯度分别为92.3%、96.3%和94.2%。这些目标化合物通过电喷雾电离质谱（ESI-MS）、氢核磁共振（¹H NMR）和碳核磁共振（¹³C NMR）进行了鉴定。
