Kading Rebekah C, Biggerstaff Brad J, Young Ginger, Komar Nicholas
Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, United States of America.
PLoS One. 2014 Jun 5;9(6):e99342. doi: 10.1371/journal.pone.0099342. eCollection 2014.
Serial samples from the same individuals may be required for certain virological studies, however, some small animals cannot easily be blood-sampled. Therefore, we evaluated the use of Culex quinquefasciatus Say and Aedes albopictus Skuse mosquitoes as "biological syringes" to draw blood for virus titer determinations in small vertebrates. Groups of chicks (Gallus gallus), hamsters (Mesocricetus auratus), and house sparrows (Passer domesticus) were experimentally infected with West Nile virus (WNV) or Highlands J virus (HJV). In general, good correlation was seen between mosquito- and syringe-derived blood samples at titers ≥5.0 log10 pfu/mL serum as compared with titers <5.0 log10 pfu/mL serum for chicks, hamsters, and sparrows. Ninety-two percent (24/26) of sparrows with virus titers >105 pfu/mL serum had mosquito- and syringe-derived titers within one log of each other. Sparrow viremia profiles generated from single mosquito blood meals and syringe were not significantly different (p>0.05). This technique is valuable for assessing the roles of small vertebrates in the ecologies of arboviruses, and could be used in applications beyond virology and infectious diseases, when <10 µL of whole blood is required.
某些病毒学研究可能需要来自同一个体的系列样本，然而，一些小型动物不易进行采血。因此，我们评估了使用致倦库蚊（Culex quinquefasciatus Say）和白纹伊蚊（Aedes albopictus Skuse）作为“生物注射器”来采集血液，以测定小型脊椎动物的病毒滴度。将雏鸡（Gallus gallus）、仓鼠（Mesocricetus auratus）和家麻雀（Passer domesticus）分组，通过实验感染西尼罗河病毒（WNV）或高地J病毒（HJV）。总体而言，对于雏鸡、仓鼠和麻雀，当血清滴度≥5.0 log10 pfu/mL时，蚊源血样和注射器采集的血样之间呈现出良好的相关性，而血清滴度<5.0 log10 pfu/mL时则不然。病毒滴度>105 pfu/mL血清的麻雀中，92%（24/26）的蚊源和注射器采集的滴度相差在一个对数以内。由单次蚊血餐和注射器采集生成的麻雀病毒血症谱无显著差异（p>0.05）。这项技术对于评估小型脊椎动物在虫媒病毒生态学中的作用具有重要价值，并且当需要的全血量<10 µL时，可用于病毒学和传染病以外的其他应用。
