检测非小细胞肺癌患者外周血中 TregFoxP3(+)和 Th17 细胞的意义。

Detection and significance of TregFoxP3(+) and Th17 cells in peripheral blood of non-small cell lung cancer patients.

机构信息

Department of Oncology, Qianfoshan Hospital, Shandong University, Jinan, P.R. China.

Institute of Basic Medical Sciences and Key Laboratory of Cardiovascular Proteomics of Shandong Province, Qilu Hospital, Shandong University, Jinan, P.R. China.

出版信息

Arch Med Sci. 2014 May 12;10(2):232-9. doi: 10.5114/aoms.2014.42573. Epub 2014 May 13.

INTRODUCTION

The aim of this study was to explore the relationships between TregFoxP3(+) cells and Th17 cells and occurrence of lung cancer.

MATERIAL AND METHODS

The proportions of TregFoxP3(+) and Th17 cells, the expression of FoxP3 and RORγt mRNA, and the levels of related cell factors such as transforming growth factor-β (TGF-β), interleukin IL-17 (IL-17) and IL-23 were determined respectively by flow cytometry analysis, real-time-polymerase chain reaction (PCR), and ELISA in peripheral blood of 18 healthy people and 26 patients with non-small cell lung cancer (NSCLC).

RESULTS

The levels of TregFoxP3(+) and Th17, expression of FoxP3 and RORγt mRNA, and ratios of TregFoxP3(+)/Th17 and FoxP3/RORγt in peripheral blood with NSCLC were higher than those in healthy controls (p < 0.05). The proportion of Th17 cells from NSCLC patients was positively correlated with that of TregFoxP3(+) (r = 0.81, p < 0.05). The receiver-operating characteristic (ROC) curve demonstrates that the increased level of TregFoxP3(+)/Th17 in the peripheral blood may be a useful indicator in early diagnosis of non-small cell lung carcinoma. The TregFoxP3(+)/Th17 and FoxP3/RORγt levels for patients in stage IV were higher than those of patients in stages I, II, and III (p < 0.05). The levels of TGF-β, IL-17, and IL-23 were higher in NSCLC patients than those in healthy controls.

CONCLUSIONS

The results suggest that ratios of Treg/Th17 correlate with the stage of NSCLC.

简介

本研究旨在探讨调节性 T 细胞 FoxP3(+)细胞与 Th17 细胞与肺癌发生的关系。

材料与方法

采用流式细胞术分析、实时聚合酶链反应（PCR）和酶联免疫吸附试验（ELISA）分别检测外周血中 18 名健康人和 26 名非小细胞肺癌（NSCLC）患者的 TregFoxP3(+)和 Th17 细胞比例、FoxP3 和 RORγt mRNA 表达以及转化生长因子-β（TGF-β）、白细胞介素-17（IL-17）和白细胞介素-23（IL-23）等相关细胞因子水平。

结果

肺癌患者外周血 TregFoxP3(+)和 Th17 细胞水平、FoxP3 和 RORγt mRNA 表达及 TregFoxP3(+)/Th17 和 FoxP3/RORγt 比值均高于健康对照组（p<0.05）。肺癌患者 Th17 细胞比例与 TregFoxP3(+)呈正相关（r=0.81，p<0.05）。受试者工作特征（ROC）曲线表明，外周血 TregFoxP3(+)/Th17 水平升高可能是早期诊断非小细胞肺癌的有用指标。IV 期患者 TregFoxP3(+)/Th17 和 FoxP3/RORγt 水平高于 I、II、III 期患者（p<0.05）。肺癌患者 TGF-β、IL-17 和 IL-23 水平高于健康对照组。