Department of Pulmonary, Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, People's Republic of China.
Med Oncol. 2013 Mar;30(1):461. doi: 10.1007/s12032-013-0461-7. Epub 2013 Jan 20.
It has been reported that the imbalance of T helper IL-17-producing cells (Th17 cells)/regulatory T cells (Tregs) was closely associated with advanced tumor growth and poor prognosis in lung cancer, but little is known about whether this imbalance is existed in the peripheral blood of patients with non-small cell lung cancer (NSCLC). Here, the percentage of Treg and Th17 cells, and the expression of transcription factors RORγt and FoxP3 were studied by flow cytometry, real-time PCR, and Western blotting, respectively. Levels of serum cytokines (IL-6, IL-17, IL-10, and TGF-β1) were analyzed by ELISA. The results showed that there was an increase in the percentage of Tregs, the mRNA and protein expression of FoxP3, the level of Treg-related cytokines in patients with NSCLC while compared to that in healthy patients. Moreover, the percentage of Th17 cells, RORγt mRNA, and protein expression were decreased in peripheral blood of NSCLC patients. In addition, Th17/Treg ratio was decreased in lung cancer patients when compared with healthy patients and Th17/Treg ratio was negatively correlated with the stages. Our results indicate that the Th17/Treg balance is abnormal in peripheral blood, which may be important in the development of NSCLC.
据报道，辅助性 T 细胞 17 型（Th17 细胞）/调节性 T 细胞（Tregs）的失衡与肺癌的肿瘤进展和预后不良密切相关，但目前尚不清楚这种失衡是否存在于非小细胞肺癌（NSCLC）患者的外周血中。在这里，通过流式细胞术、实时 PCR 和 Western blot 分别研究了 Treg 和 Th17 细胞的比例、转录因子 RORγt 和 FoxP3 的表达。通过 ELISA 分析了血清细胞因子（IL-6、IL-17、IL-10 和 TGF-β1）的水平。结果表明，与健康患者相比，NSCLC 患者外周血中 Tregs 的比例、FoxP3 的 mRNA 和蛋白表达、Treg 相关细胞因子的水平均升高。此外，NSCLC 患者外周血中 Th17 细胞的比例、RORγt mRNA 和蛋白表达降低。此外，与健康患者相比，肺癌患者的 Th17/Treg 比值降低，且 Th17/Treg 比值与分期呈负相关。我们的结果表明，外周血中的 Th17/Treg 平衡异常，这可能在 NSCLC 的发生发展中起重要作用。
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