Impact of Constant Versus Fluctuating Temperatures on the Development and Life History Parameters of (Diptera: Calliphoridae).

Necrophagous fly species are commonly used as forensic tools to estimate the minimum postmortem interval (PMI). Many researchers raised necrophagous flies under constant temperature regimes to collect their developmental data. However, in most forensic cases, the ambient temperature fluctuates. In order to investigate a forensically important fly developmental mode (the Isomegalen diagram, Isomorphen diagram and Thermal summation models) and make comparisons of the developmental rate between constant temperatures and fluctuating temperatures, we used (Diptera: Calliphoridae) to investigate the life history parameters at eight constant temperatures ranging from 8 to 36 °C. We also compared developmental rate of in three groups of constant versus fluctuating temperatures: 8 °C vs. 6-12 °C, 12 °C vs. 10-16 °C, and 16 °C vs. 14-20 °C. Our data showed that is cold tolerant with a mean (±SE) development threshold temperature () of 3.41 ± 0.48 °C and a thermal summation constant () of 8125.2 ± 288.4-degree hours. The three groups subjected to fluctuating temperatures took longer to develop compared to those developing in constant temperatures when simulated in a model. These results not only provide detailed developmental data for the use of in the estimation of the PMI, but also indicate that ambient temperature fluctuation must be taken into consideration for the use of all necrophagous fly species.