Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, India.
Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, India.
Pharmacol Rep. 2014 Feb;66(1):1-9. doi: 10.1016/j.pharep.2013.06.001. Epub 2014 Jan 30.
In the present study, antidepressant-like activity of palmatine was evaluated in unstressed and stressed young male Swiss albino mice.
The animals were subjected to unpredictable mild stress daily for 21 successive days to induce depression-like behavior. Palmatine (0.25, 0.5, 1 mg/kg, ip) was administered for 21 successive days to unstressed and stressed mice. The antidepressant-like activity was evaluated using the tail suspension test, forced swim test and sucrose preference test.
Palmatine (0.5 and 1 mg/kg, ip) significantly decreased immobility periods of unstressed and stressed mice in the forced swim test and tail suspension test, thus indicating its significant antidepressant-like activity. Only the highest dose (1 mg/kg) of palmatine significantly reversed the stress-induced decrease in sucrose preference. There was no significant effect on locomotor activity of the mice by palmatine and fluoxetine. The antidepressant-like activity of palmatine was found to be comparable to fluoxetine (10 mg/kg) administered for successive 21 days. Palmatine (0.5 and 1 mg/kg, ip) significantly reversed the stress-induced increase in brain catalase levels, MAO-A activity, lipid peroxidation, plasma nitrite and corticosterone levels.
Palmatine showed significant antidepressant-like activity in unstressed and stressed mice probably through inhibition of MAO-A activity, decrease in plasma nitrite levels and due to its antioxidant activity. In addition, palmatine also showed antidepressant-like activity in stressed mice probably through decrease in plasma corticosterone levels.
在本研究中，评估了小檗碱在未应激和应激的年轻雄性瑞士白化小鼠中的抗抑郁样活性。
动物每天接受不可预测的轻度应激，连续 21 天，以诱导抑郁样行为。连续 21 天给未应激和应激的小鼠腹腔注射小檗碱（0.25、0.5、1mg/kg）。使用悬尾试验、强迫游泳试验和蔗糖偏好试验评估抗抑郁样活性。
小檗碱（0.5 和 1mg/kg，ip）显著减少未应激和应激小鼠在强迫游泳试验和悬尾试验中的不动时间，表明其具有显著的抗抑郁样活性。只有最高剂量（1mg/kg）的小檗碱显著逆转了应激引起的蔗糖偏好降低。小檗碱和氟西汀对小鼠的运动活性没有显著影响。小檗碱的抗抑郁样活性与连续 21 天给予氟西汀（10mg/kg）相当。小檗碱（0.5 和 1mg/kg，ip）显著逆转了应激引起的大脑过氧化氢酶水平、MAO-A 活性、脂质过氧化、血浆亚硝酸盐和皮质酮水平的升高。
小檗碱在未应激和应激的小鼠中表现出显著的抗抑郁样活性，可能是通过抑制 MAO-A 活性、降低血浆亚硝酸盐水平以及其抗氧化活性。此外，小檗碱还通过降低应激小鼠的血浆皮质酮水平表现出抗抑郁样活性。
