Tamburini Beth A, Burchill Matthew A, Kedl Ross M
Integrated Department of Immunology, University of Colorado, Denver, 1400 Jackson St, K825, Denver, Colorado 80206, USA.
Nat Commun. 2014 Jun 6;5:3989. doi: 10.1038/ncomms4989.
Antigen derived from viral infections with influenza and vesicular stomatitis virus can persist after resolution of infection. Here we show that antigen can similarly persist for weeks following viral challenge and vaccination. Antigen is captured by lymphatic endothelial cells (LECs) under conditions that induce LEC proliferation. Consistent with published data showing that viral antigen persistence impacts the function of circulating memory T cells, we find that vaccine-elicited antigen persistence, found on LECs, positively influences the degree of protective immunity provided by circulating memory CD8(+) T cells. The coupling of LEC proliferation and antigen capture identifies a mechanism by which the LECs store, or 'archive', antigens for extended periods of time after antigen challenge, thereby increasing IFNγ/IL-2 production and enhancing protection against infection. These findings therefore have the potential to have an impact on future vaccination strategies and our understanding of the role for persisting antigen in both vaccine and infectious settings.
源自流感病毒感染和水疱性口炎病毒感染的抗原在感染消退后仍可存留。在此我们表明，病毒攻击和接种疫苗后，抗原同样可持续存留数周。在诱导淋巴管内皮细胞（LEC）增殖的条件下，抗原被LEC捕获。与已发表的数据一致，这些数据表明病毒抗原的存留会影响循环记忆T细胞的功能，我们发现存在于LEC上的疫苗诱导的抗原存留对循环记忆CD8(+) T细胞提供的保护性免疫程度有积极影响。LEC增殖与抗原捕获的耦合确定了一种机制，通过该机制，LEC在抗原攻击后可长时间储存或“存档”抗原，从而增加IFNγ/IL-2的产生并增强抗感染能力。因此，这些发现有可能对未来的疫苗接种策略以及我们对存留抗原在疫苗和感染环境中的作用的理解产生影响。
