Lao Jeemeng, Oikawa Ai, Bromley Jennifer R, McInerney Peter, Suttangkakul Anongpat, Smith-Moritz Andreia M, Plahar Hector, Chiu Tsan-Yu, González Fernández-Niño Susana M, Ebert Berit, Yang Fan, Christiansen Katy M, Hansen Sara F, Stonebloom Solomon, Adams Paul D, Ronald Pamela C, Hillson Nathan J, Hadi Masood Z, Vega-Sánchez Miguel E, Loqué Dominique, Scheller Henrik V, Heazlewood Joshua L
Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Plant J. 2014 Aug;79(3):517-29. doi: 10.1111/tpj.12577. Epub 2014 Jul 9.
The glycosyltransferases (GTs) are an important and functionally diverse family of enzymes involved in glycan and glycoside biosynthesis. Plants have evolved large families of GTs which undertake the array of glycosylation reactions that occur during plant development and growth. Based on the Carbohydrate-Active enZymes (CAZy) database, the genome of the reference plant Arabidopsis thaliana codes for over 450 GTs, while the rice genome (Oryza sativa) contains over 600 members. Collectively, GTs from these reference plants can be classified into over 40 distinct GT families. Although these enzymes are involved in many important plant specific processes such as cell-wall and secondary metabolite biosynthesis, few have been functionally characterized. We have sought to develop a plant GTs clone resource that will enable functional genomic approaches to be undertaken by the plant research community. In total, 403 (88%) of CAZy defined Arabidopsis GTs have been cloned, while 96 (15%) of the GTs coded by rice have been cloned. The collection resulted in the update of a number of Arabidopsis GT gene models. The clones represent full-length coding sequences without termination codons and are Gateway® compatible. To demonstrate the utility of this JBEI GT Collection, a set of efficient particle bombardment plasmids (pBullet) was also constructed with markers for the endomembrane. The utility of the pBullet collection was demonstrated by localizing all members of the Arabidopsis GT14 family to the Golgi apparatus or the endoplasmic reticulum (ER). Updates to these resources are available at the JBEI GT Collection website http://www.addgene.org/.
糖基转移酶（GTs）是参与聚糖和糖苷生物合成的一类重要且功能多样的酶。植物进化出了庞大的GTs家族，它们负责植物发育和生长过程中发生的一系列糖基化反应。根据碳水化合物活性酶（CAZy）数据库，参考植物拟南芥的基因组编码超过450种GTs，而水稻基因组（Oryza sativa）包含超过600个成员。总体而言，来自这些参考植物的GTs可分为40多个不同的GT家族。尽管这些酶参与了许多重要的植物特异性过程，如细胞壁和次生代谢物的生物合成，但很少有酶的功能得到表征。我们试图开发一种植物GTs克隆资源，使植物研究界能够采用功能基因组学方法。总共克隆了CAZy定义的403种（88%）拟南芥GTs，而水稻编码的GTs中有96种（15%）已被克隆。该收集结果更新了一些拟南芥GT基因模型。这些克隆代表没有终止密码子的全长编码序列，并且与Gateway®兼容。为了证明JBEI GT文库的实用性，还构建了一组带有内膜标记的高效粒子轰击质粒（pBullet）。通过将拟南芥GT14家族的所有成员定位到高尔基体或内质网（ER），证明了pBullet文库的实用性。这些资源的更新可在JBEI GT文库网站http://www.addgene.org/上获取。
