Han Yi-Long, Xu Qin, Lu Zhi-Qian, Wang Jin-Ye
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
Department of Cardio-Thoracic Surgery, Shanghai No.6 People's Hospital Affiliated Shanghai Jiao Tong University, Shanghai 200233, China.
Colloids Surf B Biointerfaces. 2014 Aug 1;120:55-62. doi: 10.1016/j.colsurfb.2014.04.019. Epub 2014 May 2.
In our previous studies, zein has shown good cell compatibility in both films and porous scaffolds. To use the zein film in microfluidic devices or as a cell culture substrate, swelling and excessive degradation should be avoided. Moreover, the film should be transparent. In this study, we found that the zein film could maintain good transparency even after swelling when it was treated for 20min at 121°C, 100% relative humidity and 103.4kPa, especially for the zein film without the addition of a plasticizer. FT-IR and XRD analysis showed that the ratio of α-helix of zein structure decreased and the ratio of its β-sheet increased. Proliferation of NIH 3T3 cells on the transparent zein film was as cytocompatible as the untreated one and the cell culture plate. The improved transparency of zein films after swelling will expand the application field of zein.
在我们之前的研究中，玉米醇溶蛋白在薄膜和多孔支架中均表现出良好的细胞相容性。为了在微流控装置中使用玉米醇溶蛋白薄膜或作为细胞培养底物，应避免其膨胀和过度降解。此外，该薄膜应具有透明性。在本研究中，我们发现，当玉米醇溶蛋白薄膜在121°C、100%相对湿度和103.4kPa条件下处理20分钟后，即使在膨胀后仍能保持良好的透明性，尤其是对于未添加增塑剂的玉米醇溶蛋白薄膜。傅里叶变换红外光谱（FT-IR）和X射线衍射（XRD）分析表明，玉米醇溶蛋白结构的α-螺旋比例降低，β-折叠比例增加。NIH 3T3细胞在透明玉米醇溶蛋白薄膜上的增殖与未处理的薄膜和细胞培养板具有相同的细胞相容性。玉米醇溶蛋白薄膜膨胀后透明度的提高将扩大其应用领域。
