School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Department of Cardio-thoracic Surgery, Shanghai No. 6 People Hospital, Affiliated Shanghai Jiao Tong University, Shanghai 200233, China.
Colloids Surf B Biointerfaces. 2013 Nov 1;111:479-85. doi: 10.1016/j.colsurfb.2013.06.042. Epub 2013 Jul 5.
Vascular implants after implantation need to improve the ability of cells to withstand flow-shear stress. As such, we want to test whether zein films could improve the flow-shear stress resistance of cells by control of their surface morphology. We chose Collagen, poly L-lactic acid (PLLA) and three types of zein as the coating films and evaluated the flow-shear stress resistance of NIH3T3, and EA.hy926 on these respective films. The results showed that the retention of two cell lines on Collagen film was better than PLLA and zein films. The cell retention of EA.hy926 on Zein 3 film with higher roughness was better than Zein 1 film with a flat surface in the first 2h. The cell retention of NIH3T3 on a rougher surface was always better than the smoother one under flow-shear stress condition for 6h. Observation of cell morphologies showed that the aspect ratio changed significantly for NIH3T3 cells upon flow-shear stress condition, as shown by reduced numbers of pseudopodia, increased cell rounding and shrinkage. Zein 3 film with higher roughness improved the flow-shear stress resistance of cells and might be used in vascular implant coatings.
血管植入物植入后需要提高细胞承受流切应力的能力。因此，我们希望通过控制其表面形态来测试玉米醇溶蛋白膜是否可以提高细胞的流切应力阻力。我们选择胶原蛋白、聚 L-乳酸 (PLLA) 和三种玉米醇溶蛋白作为涂层膜，并在这些各自的膜上评估 NIH3T3 和 EA.hy926 的流切应力阻力。结果表明，两种细胞系在胶原蛋白膜上的保留率优于 PLLA 和玉米醇溶蛋白膜。在最初的 2 小时内，粗糙度较高的 Zein 3 膜上的 EA.hy926 细胞保留率优于表面平整的 Zein 1 膜。在流切应力条件下，NIH3T3 细胞在较粗糙表面上的保留率始终优于较光滑表面上的保留率，持续 6 小时。细胞形态观察表明，NIH3T3 细胞在流切应力条件下的纵横比发生了显著变化，表现为伪足数量减少、细胞圆化和收缩增加。具有较高粗糙度的 Zein 3 膜提高了细胞的流切应力阻力，可能用于血管植入物涂层。
