Gupta Meetu, Ahmad Mohd Anwar
Ecotoxicogenomics Lab, Department of Biotechnology, Jamia Millia Islamia, New Delhi-110025, India.
Ecotoxicogenomics Lab, Department of Biotechnology, Jamia Millia Islamia, New Delhi-110025, India.
Ecotoxicol Environ Saf. 2014 Sep;107:46-54. doi: 10.1016/j.ecoenv.2014.04.030. Epub 2014 Jun 4.
To study the differential response in two rice genotypes (PB1 and IR-64), hydroponically grown 14 days old plants were exposed to 50, 150 and 300µM As(V) for 24 and 96h. Accumulation of As was not significantly higher in PB1 variety except at higher concentration (300μM) and duration (96h), but up regulation of gene transcripts were higher as compared to IR-64. Inhibition in seed germination, root-shoot length, chlorophyll and protein content was observed in both varieties with increasing concentration and exposure time. PB1 variety was found more capable to detoxify As(V) through induction of antioxidant defense system and other stress related parameters (cysteine, proline content). SDS-PAGE and semi quantitative RT-PCR analysis showed significant changes in protein profile and gene expression analysis. The results suggests that various studied parameters and transcripts accumulation showed a combinatorial type of tolerance mechanism in PB1 variety to provide better protection against As(V) stress.
为研究两种水稻基因型（PB1和IR - 64）的差异反应，将水培14天的植株暴露于50、150和300µM的砷（V）中24小时和96小时。除了在较高浓度（300μM）和较长时间（96小时）外，PB1品种中砷的积累量并没有显著高于其他品种，但与IR - 64相比，其基因转录本的上调程度更高。随着浓度和暴露时间的增加，两个品种的种子萌发、根和茎的长度、叶绿素和蛋白质含量均受到抑制。发现PB1品种更有能力通过诱导抗氧化防御系统和其他与胁迫相关的参数（半胱氨酸、脯氨酸含量）来解毒砷（V）。SDS - PAGE和半定量RT - PCR分析表明蛋白质谱和基因表达分析有显著变化。结果表明，在PB1品种中，各种研究参数和转录本积累显示出一种组合型的耐受机制，以提供更好的保护来抵御砷（V）胁迫。
