Ozkul Yilmaz, Songu Murat, Basoglu Mehmet Sinan, Ozturkcan Sedat, Katilmis Huseyin
From the Department of Otorhinolaryngology, Izmir Katip Celebi University, Ataturk Training and Research Hospital, Izmir, Turkey.
J Craniofac Surg. 2014 Jul;25(4):1515-8. doi: 10.1097/SCS.0000000000000881.
The aim of our study was to determine the effectiveness of intratympanic α-lipoic acid injection as an otoprotective agent against cisplatin-induced ototoxicity in guinea pigs. Twenty-four adult male albino guinea pigs with normal hearing were divided into 4 groups. The guinea pigs received intraperitoneal cisplatin in group 1, intraperitoneal cisplatin and intratympanic α-lipoic acid in group 2, intratympanic α-lipoic acid in group 3, as well as intraperitoneal cisplatin and intratympanic saline in group 4. Distortion-product otoacoustic emission measurements were obtained for both ears at the following time points: before administration (baseline recording) and on day 3 (72 h later). In group 1 (cisplatin), significant deterioration was observed at all frequencies on day 3 (P < 0.05). In group 2 (cisplatin + α-lipoic acid), deterioration was observed at all frequencies on day 3; however, this deterioration did not reach a statistical significance (P > 0.05). In group 3 (α-lipoic acid), no significant difference was observed between baseline and day 3 (P > 0.05). In group 4 (cisplatin + saline), deterioration was observed at all frequencies on day 3; however, this deterioration did not reach a statistical significance (P > 0.05). Cisplatin-induced hearing loss in the guinea pigs may be limited to some extent by the concomitant use of α-lipoic acid. Dose-dependent changes in the possible effects of α-lipoic acid need further investigation. Future morphologic studies may contribute to expose clearly the protective effect of α-lipoic acid.
我们研究的目的是确定鼓室内注射α-硫辛酸作为一种耳保护剂，预防顺铂诱导的豚鼠耳毒性的有效性。将24只听力正常的成年雄性白化豚鼠分为4组。第1组豚鼠接受腹腔注射顺铂，第2组接受腹腔注射顺铂和鼓室内注射α-硫辛酸，第3组接受鼓室内注射α-硫辛酸，第4组接受腹腔注射顺铂和鼓室内注射生理盐水。在以下时间点对双耳进行畸变产物耳声发射测量：给药前（基线记录）和第3天（72小时后）。在第1组（顺铂组），第3天所有频率均观察到明显恶化（P<0.05）。在第2组（顺铂+α-硫辛酸组），第3天所有频率均观察到恶化；然而，这种恶化未达到统计学显著性（P>0.05）。在第3组（α-硫辛酸组），基线和第3天之间未观察到显著差异（P>0.05）。在第4组（顺铂+生理盐水组），第3天所有频率均观察到恶化；然而，这种恶化未达到统计学显著性（P>0.05）。顺铂诱导的豚鼠听力损失在一定程度上可能会因同时使用α-硫辛酸而受到限制。α-硫辛酸可能作用的剂量依赖性变化需要进一步研究。未来的形态学研究可能有助于更清楚地揭示α-硫辛酸的保护作用。
