Sun Haitao, Hou Zongliu, Yang Huaqiang, Meng Mingyao, Li Peng, Zou Qingjian, Yang Lujun, Chen Yuxin, Chai Huihui, Zhong Huilin, Yang Zara Zhuyun, Zhao Jing, Lai Liangxue, Jiang Xiaodan, Xiao Zhicheng
Department of Neurosurgery, The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China.
Cell Tissue Res. 2014 Sep;357(3):571-82. doi: 10.1007/s00441-014-1903-z. Epub 2014 Jun 8.
Amyotrophic lateral sclerosis (ALS) is an adult-onset progressive neurodegenerative disease involving degeneration of motor neurons in the central nervous system. Stem cell treatment is a potential therapy for this fatal disorder. The human amniotic membrane (HAM), an extremely rich and easily accessible tissue, has been proposed as an attractive material in cellular therapy and regenerative medicine because of its advantageous characteristics. In the present study, we evaluate the long-term effects of a cellular treatment by intravenous administration of human amniotic mesenchymal stem cells (hAMSCs) derived from HAM into a hSOD1(G93A) mouse model. The mice received systemic administration of hAMSCs or phosphate-buffered saline (PBS) at the onset, progression and symptomatic stages of the disease. hAMSCs were detected in the spinal cord at the final stage of the disease, in the form of isolates or clusters and were negative for β-tubulin III and GFAP. Compared with the treatment with PBS, multiple hAMSC transplantations significantly retarded disease progression, extended survival, improved motor function, prevented motor neuron loss and decreased neuroinflammation in mice. These findings demonstrate that hAMSC transplantation is a promising cellular treatment for ALS.
肌萎缩侧索硬化症（ALS）是一种成年发病的进行性神经退行性疾病，涉及中枢神经系统运动神经元的退化。干细胞治疗是这种致命疾病的一种潜在疗法。人羊膜（HAM）是一种极其丰富且易于获取的组织，因其具有有利特性，已被提议作为细胞治疗和再生医学中有吸引力的材料。在本研究中，我们评估了通过静脉注射源自HAM的人羊膜间充质干细胞（hAMSCs）对hSOD1（G93A）小鼠模型进行细胞治疗的长期效果。在疾病的发病期、进展期和症状期，小鼠接受了hAMSCs或磷酸盐缓冲盐水（PBS）的全身给药。在疾病的最后阶段，在脊髓中检测到hAMSCs，呈分离状态或簇状，且β-微管蛋白III和胶质纤维酸性蛋白呈阴性。与PBS治疗相比，多次hAMSC移植显著延缓了疾病进展，延长了生存期，改善了运动功能，防止了运动神经元丢失，并减少了小鼠的神经炎症。这些发现表明，hAMSC移植是一种有前景的ALS细胞治疗方法。
