Rodríguez-Gabriel Miguel A
Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Científicas (UAM-CSIC), Universidad Autónoma de Madrid, Cantoblanco, Madrid, 28049, Spain,
Methods Mol Biol. 2014;1170:383-92. doi: 10.1007/978-1-4939-0888-2_20.
Stress leads to multiple changes in the physiology of the cell. One of the most important is the adaptation of the cell cycle to the changing conditions of the environment. Cellular responses after stress can be followed by cellular synchronization previous to the insult. In this chapter, we use centrifugal elutriation to synchronize Schizosaccharomyces pombe cells and outline methods to investigate the hallmarks of cell cycle progression upon stress. These include analyses of cyclin-dependent kinase phosphorylation and cell size change.
应激会导致细胞生理发生多种变化。其中最重要的变化之一是细胞周期适应不断变化的环境条件。应激后的细胞反应可在损伤前通过细胞同步化来追踪。在本章中，我们使用离心淘析法使粟酒裂殖酵母细胞同步化，并概述研究应激时细胞周期进程特征的方法。这些方法包括对细胞周期蛋白依赖性激酶磷酸化和细胞大小变化的分析。
